CAJ | 학술논문

设置3个种植处理(玉米单作、马铃薯单作和玉米马铃薯间作)，通过田间试验和盆栽试验，采用Biolog技术分析了玉米马铃薯根际微生物的群落结构和功能多样性的变化情况。结果表明，与单作相比，间作处理使玉米和马铃薯根际微生物对31种碳源的平均利用率(AWCD)分别增加17.36%、7.38%和3.76%、32.21%，其中间作马铃薯的AWCD值最高，单作玉米最低。间作玉米和间作马铃薯根际微生物群落的Shannon指数(H)、Simpson指数(D)、均匀度指数(E)、丰富度指数(S)均高于单作，但在间作和单作之间差异不显著。根际微生物对6类碳源的利用强度以糖类、羧酸类和氨基酸最高，并存在一定的差异。主成分和聚类分析表明，玉米与马铃薯间作改变了根际微生物的群落组成，糖类、羧酸类和氨基酸类碳源是区分单间作处理差异的敏感碳源。说明玉米马铃薯间作改变了根际微生物群落结构组成，提高了根际微生物群落功能多样性。
설치3개충식처리(옥미단작、마령서단작화옥미마령서간작)，통과전간시험화분재시험，채용Biolog기술분석료옥미마령서근제미생물적군락결구화공능다양성적변화정황。결과표명，여단작상비，간작처리사옥미화마령서근제미생물대31충탄원적평균이용솔(AWCD)분별증가17.36%、7.38%화3.76%、32.21%，기중간작마령서적AWCD치최고，단작옥미최저。간작옥미화간작마령서근제미생물군락적Shannon지수(H)、Simpson지수(D)、균균도지수(E)、봉부도지수(S)균고우단작，단재간작화단작지간차이불현저。근제미생물대6류탄원적이용강도이당류、최산류화안기산최고，병존재일정적차이。주성분화취류분석표명，옥미여마령서간작개변료근제미생물적군락조성，당류、최산류화안기산류탄원시구분단간작처리차이적민감탄원。설명옥미마령서간작개변료근제미생물군락결구조성，제고료근제미생물군락공능다양성。
Field and pot experiments were conducted with three planting treatments (maize monocropping, potato monocropping, maize, and potato intercropping) to investigate the changes of composition and function diversity of rhizosphere microbial com-munity of maize and potato using Biolog technique. The results indicated that compared with monocropping, the average well color development (AWCD, utilization rate of 31 carbon sources) of rhizosphere microbes of intercropped maize and potato was increased by 17.36%, 7.38% and 3.76%, 32.21% respectively, among the intercropping and monocropping crops, the AWCD value of intercropped potato was the highest and the monocropped maize was the lowest. Functional diversity index of Shannon index (H), Simpson index (D), Evenness index (E), and Richness index (S) were higher in intercropping rhizosphere soil than these in the monocropping systems, but no significant difference. Among six types of carbon sources used by the rhizospheric microbes, carbohydrates, amino acids and carboxylic acids, had higher utilization rates with definite differences. Principal com-ponent analysis (PCA) and cluster analysis demonstrated that the amino acids, carbohydrates and carboxylic acids were the sensi-tive carbon sources for differentiating the changes of the microbial community induced by monocropping and intercropping. This study showed that intercropping can change the composition and enhance functional diversity of the microbial community in the rhizosphere of maize and potato.