CAJ | 학술논문

WRKY 转录因子广泛参与植物抗逆胁迫反应，全面分析挖掘小麦 A 染色体组供体物种乌拉尔图小麦中的WRKY转录因子，对进一步挖掘分析六倍体小麦WRKY转录因子的分子功能具有重要意义。本研究通过生物信息学分析，在乌拉尔图小麦基因组数据中鉴定得到62条全长 WRKY转录因子，其中14条能被准确定位在染色体上，并发现2对WRKY转录因子发生了复制；通过进化树分析，发现62条WRKY转录因子被分为8个小亚族，且小亚族内部的基因结构相对保守。通过荧光定量分析所选基因在非生物胁迫下的表达模式，筛选到2条在不同非生物胁迫下均上调表达的WRKY转录因子，为进一步分析其功能打下基础。
WRKY 전록인자엄범삼여식물항역협박반응，전면분석알굴소맥 A 염색체조공체물충오랍이도소맥중적WRKY전록인자，대진일보알굴분석륙배체소맥WRKY전록인자적분자공능구유중요의의。본연구통과생물신식학분석，재오랍이도소맥기인조수거중감정득도62조전장 WRKY전록인자，기중14조능피준학정위재염색체상，병발현2대WRKY전록인자발생료복제；통과진화수분석，발현62조WRKY전록인자피분위8개소아족，차소아족내부적기인결구상대보수。통과형광정량분석소선기인재비생물협박하적표체모식，사선도2조재불동비생물협박하균상조표체적WRKY전록인자，위진일보분석기공능타하기출。
WRKY transcription factors have been found to be involved in the processes responding to various abiotic and biotic stresses in plants. The knowledge on WRKY transcription factors inTriticum urartu (AA) will facilitate the function study of WRKY transcription factors in hexaploid wheat (Triticum aestivum, AABBDD). In this study, 62 WRKY transcription factors with full length of coding sequence (CDS) were selected fromTriticum urartu genome through bioinformatic analyses, in which 14 could be located on specific chromosomes and two pairs had been duplicated. These WRKY transcription factors were divided into eight subgroups by phylogenetic analysis and the exon–intron structure in individual subgroups was relatively conserved. Two WRKY transcription factors were selected for function validation, and their expressions consistently increased under diverse abiotic stresses by qRT-PCR assay.