CAJ | 학술논문

以丰产性好、抗旱力强的栽培大豆晋豆23为母本,山西农家品种半野生大豆灰布支黑豆为父本杂交衍生的447个RIL作为供试群体。将亲本及447个家系分别于2011、2012和2013年采用随机试验种植,按照标准测量叶长、叶宽和叶柄长3个性状,并于2012年8月1日和8月8日和2013年8月2日和8月9日各测量1次叶绿素含量。采用QTLNETwork 2.0混合线性模型分析方法和主基因+多基因混合遗传分离分析法,对大豆叶片性状和叶绿素含量进行遗传分析和QTL间的上位性和环境互作效应研究。结果表明,叶长受2对加性-加性×加性上位性混合主基因控制,叶宽受3对等效主基因控制,叶柄长受4对加性-加性×加性上位性主基因控制,叶绿素含量受4对加性主基因控制；检测到10个与叶长、叶宽、叶柄长和叶绿素含量相关的QTL,分别位于A1、A2、C2、H_1、L和O染色体。其中2个叶长QTL分别位于C2和L染色体,是2对加性×加性上位互作效应及环境互作效应QTL；3个叶宽加性与环境互作QTL分别位于A2、C2和O染色体；2个叶柄长QTL分别位于L和O染色体；3个叶绿素含量QTL分别位于A1、C2和H_1染色体。叶片性状和叶绿素含量的遗传机制较复杂,加性效应、加性×加性上位互作效应及环境互作效应是大豆叶片性状和叶绿素含量的重要遗传基础。建议大豆分子标记辅助育种中,一方面要考虑起主要作用的QTL,另一方面要注重上位性QTL的影响,这对于性状的遗传和稳定表达具有积极的意义。
이봉산성호、항한력강적재배대두진두23위모본,산서농가품충반야생대두회포지흑두위부본잡교연생적447개RIL작위공시군체。장친본급447개가계분별우2011、2012화2013년채용수궤시험충식,안조표준측량협장、협관화협병장3개성상,병우2012년8월1일화8월8일화2013년8월2일화8월9일각측량1차협록소함량。채용QTLNETwork 2.0혼합선성모형분석방법화주기인+다기인혼합유전분리분석법,대대두협편성상화협록소함량진행유전분석화QTL간적상위성화배경호작효응연구。결과표명,협장수2대가성-가성×가성상위성혼합주기인공제,협관수3대등효주기인공제,협병장수4대가성-가성×가성상위성주기인공제,협록소함량수4대가성주기인공제；검측도10개여협장、협관、협병장화협록소함량상관적QTL,분별위우A1、A2、C2、H_1、L화O염색체。기중2개협장QTL분별위우C2화L염색체,시2대가성×가성상위호작효응급배경호작효응QTL；3개협관가성여배경호작QTL분별위우A2、C2화O염색체；2개협병장QTL분별위우L화O염색체；3개협록소함량QTL분별위우A1、C2화H_1염색체。협편성상화협록소함량적유전궤제교복잡,가성효응、가성×가성상위호작효응급배경호작효응시대두협편성상화협록소함량적중요유전기출。건의대두분자표기보조육충중,일방면요고필기주요작용적QTL,령일방면요주중상위성QTL적영향,저대우성상적유전화은정표체구유적겁적의의。
An RIL population containing 447 lines, derived from a cross of cultivar Jingdou 23 × Huibuzhiheidou, as well as their parents were used to analyze inheritance and detect epistatic effects, and QTL × environment (QE) interactions related to leaf traits and leaf chlorophyll content (CC) in soybean using major gene plus polygene mixed inheritance analysis and composite interval mapping (QTL NETwork 2.0). The leaf traits including leaf length (LL), leaf width (LW), leaf stalk length (LSL) were evaluated in 2011, 2012, and 2013, as well as CC was detected on Aug. 1 and Aug. 8, 2012, and on Aug. 2 and Aug. 9, 2013. LL was found to be controlled by two pairs of additive-additive by additive epistatic hybrid main genes, LW was found to be con-trolled by three pairs of equivalent main genes, LSL was found to be controlled by four pairs of additive-additive by additive epistatic major genes, CC was controlled by four pairs of additive major genes. Ten QTLs for LL, LW, LSL, and CC were mapped on the linkage group (LG) A1, A2, C2, H_1, L, and O, respectively. Of them two QTLs for LL were mapped on LG C2 and LG L, additive by additive epistatic effect and QE interactions. Three QTLs with additive effect and QE interactions associated with LW were mapped on LG A2, C2, and O. Two QTLs for LSL were mapped on LG L and O. Three QTLs for CC were mapped on LG A1, C2, and H_1. The genetic mechanism for leaf traits and leaf chlorophyll content is more complicated containing additive ef-fect, additive × additive epistatic effect and QE interaction. It is important to consider not only to QTLs with major effects, but also to those with epistatic effects in soybean molecular marker-assisted breeding for stability of expression and inheritance of agronomic traits.