CAJ | 학술논문

水稻开花灌浆期，内外颖呈绿色，含光合色素，为阐释非叶片组织叶绿体发育的分子机制，本文对 EMS 诱变获得的新型白穗突变体wp4进行了研究。抽穗灌浆期， wp4的穗轴呈绿色，内外颖呈乳白色；内外颖叶绿体发育不健全，无类囊体结构，叶绿素a、叶绿素b和类胡萝卜素含量极显著降低。此外， wp4全生育期叶片呈淡黄色，叶绿体内基质片层较少且排列松散；与野生型相比， wp4的叶绿素a含量显著降低，叶绿素b和类胡萝卜素含量虽略低，但差异未达到显著水平。农艺性状分析发现，与野生型相比， wp4的有效穗和结实率略有增加，其他性状则略有降低，但变化均未达到显著差异水平。遗传分析表明wp4受一对隐性核基因调控，利用1200株西农1A/wp4的F2隐性群体，最终将WP4精细定位在第8染色体约79 kb的物理范围内，根据水稻基因组注释计划，该区间包含14个基因，这为WP4的功能和作用机制研究奠定了基础，也为水稻标记性状育种提供了新的资源。
수도개화관장기，내외영정록색，함광합색소，위천석비협편조직협록체발육적분자궤제，본문대 EMS 유변획득적신형백수돌변체wp4진행료연구。추수관장기， wp4적수축정록색，내외영정유백색；내외영협록체발육불건전，무류낭체결구，협록소a、협록소b화류호라복소함량겁현저강저。차외， wp4전생육기협편정담황색，협록체내기질편층교소차배렬송산；여야생형상비， wp4적협록소a함량현저강저，협록소b화류호라복소함량수략저，단차이미체도현저수평。농예성상분석발현，여야생형상비， wp4적유효수화결실솔략유증가，기타성상칙략유강저，단변화균미체도현저차이수평。유전분석표명wp4수일대은성핵기인조공，이용1200주서농1A/wp4적F2은성군체，최종장WP4정세정위재제8염색체약79 kb적물리범위내，근거수도기인조주석계화，해구간포함14개기인，저위WP4적공능화작용궤제연구전정료기출，야위수도표기성상육충제공료신적자원。
At the flowering and filling stages, rice hull appears green color and possesses photosynthetic pigment. To clear the mechanism of chloroplast development in the panicle, we identified a novel white panicle mutant from the progeny ofindica re-storer line Jinhui 10 with seeds treated by EMS and termed it aswp4. Thewp4 displayed green spike-stalks and milk-white hulls after the heading stage. The structures of chloroplast and thylakoid were severely destroyed and the contents of photosynthetic pigment decreased extremely significantly in the mutational hulls. Compared with the wild type, thewp4 displayed yellow green leaves and contained looser stromal lamellae. And the contents of chlorophylla,b, and carotenoid were all declined while only the changing of chlorophyll a led to the significantly different level in statistics. Except for effective panicles number and seed setting rate, other detected agronomic characteristics decreased slightly but the changing did not come up to the statistically significant difference. Genetic analysis indicated that the white panicle ofwp4 was controlled by a recessive nuclear gene and which was finally mapped on chromosome 8 with 79 kb physical distances according to 1200 mutational plants derived from the F2 genera-tion of Xinong1A/wp4. The restricted region contained 14 annotated genes based on Rice Genome Annotation Project. These re-sults provided a foundation for gene cloning and function analysis of theWP4. Meanwhile, the traits ofwp4 could be available in rice breeding as a morphological marker.