CAJ | 학술논문

转绿型叶色突变体是研究植物叶绿体分化与发育的基础材料。grc2是利用60Co-γ射线诱变籼型三系保持系T98B后获得的单叶独立转绿型黄化突变体。grc2植株上任一叶片刚抽出时为黄色，在生长10 d左右后变绿，具有单叶不依赖于植株特定发育阶段而独立转绿的特性。与野生型T98B相比， grc2黄化叶片的总叶绿素和叶绿素b含量显著降低，叶绿体滞留在黄化质体阶段，表明grc2可能在叶片早期发育中起关键作用。遗传分析表明， grc2受1对隐性核基因独立控制；利用源于grc2/Nipponbare的F2群体的960个突变单株，将grc2基因定位在STS标记S254与S258之间约31 kb的范围内，该区域含有5个未报道过的注释基因。这些结果为grc2的克隆及功能研究提供了重要信息。
전록형협색돌변체시연구식물협록체분화여발육적기출재료。grc2시이용60Co-γ사선유변선형삼계보지계T98B후획득적단협독립전록형황화돌변체。grc2식주상임일협편강추출시위황색，재생장10 d좌우후변록，구유단협불의뢰우식주특정발육계단이독립전록적특성。여야생형T98B상비， grc2황화협편적총협록소화협록소b함량현저강저，협록체체류재황화질체계단，표명grc2가능재협편조기발육중기관건작용。유전분석표명， grc2수1대은성핵기인독립공제；이용원우grc2/Nipponbare적F2군체적960개돌변단주，장grc2기인정위재STS표기S254여S258지간약31 kb적범위내，해구역함유5개미보도과적주석기인。저사결과위grc2적극륭급공능연구제공료중요신식。
Green revertible leaf-color mutants are basical materials for studying the mechanism of chloroplast differentiation and development. We have obtained agreen-revertible chlorina mutant namedgrc2 with every leaf greening independently, from an indica maintainer line T98B treated by60Co-γ radiation. Each leaf ofgrc2 is initially chlorotic, and then turns green after growing about 10 days. The mutantgrc2 showed a new pattern of virescence which refreshed green regardless of its plant growth stage. Compared with the wild type T98B, the total chlorophyll and chlorophyll b content reduced significantly in the yellowish leaves ofgrc2 and chloroplast remained in the etioplast stage, suggesting thatgrc2 would probably be an essential gene functioning in the development of young leaves. Genetic analysis revealed that,grc2 was controlled by a single recessive nuclear gene. The gene ofgrc2 was fine mapped between STS markers S254 and S258 with a physical interval of 31 kb on the short arm of chromosome 6, by using 960 F2 plants with mutant phenotype from a cross betweengrc2 and Nipponbare. This region contained five annotated genes that had not published. These results provides important information for studying in gene cloning and gene function ofgrc2.