江西师范大学学报(自然科学版)
江西師範大學學報(自然科學版)
강서사범대학학보(자연과학판)
JOURNAL OF JIANGXI NORMAL UNIVERSITY(NATURAL SCIENCES EDITION)
2015年
3期
246-248
,共3页
吴浪%张勇%李存雄%赵君%陈顺琴%周永丹
吳浪%張勇%李存雄%趙君%陳順琴%週永丹
오랑%장용%리존웅%조군%진순금%주영단
高效液相色谱%苦荞%多菌灵%残留
高效液相色譜%苦蕎%多菌靈%殘留
고효액상색보%고교%다균령%잔류
HPLC%buckwheat%carbendazim%residual
建立了一种分析苦荞中多菌灵残留量的高效液相色谱法( HPLC).苦荞粉粹后,用乙酸乙酯超声提取,净化、浓缩、用乙腈定容.采用配有紫外检测器的HPLC测定,以乙腈-水(体积比为65∶35)为流动相,流速为1.0 mL·min-1,检测波长为282 nm.浓度在0.025~5.000μg·mL-1范围内多菌灵的质量浓度与峰面积线性关系良好,R2为0.9999;平均回收率为83.60%~91.59%,RSD为2.11%~6.56%,方法的最低检出限(3S/N)为0.01 mg·kg-1.该方法操作简单,灵敏度、重现性良好.
建立瞭一種分析苦蕎中多菌靈殘留量的高效液相色譜法( HPLC).苦蕎粉粹後,用乙痠乙酯超聲提取,淨化、濃縮、用乙腈定容.採用配有紫外檢測器的HPLC測定,以乙腈-水(體積比為65∶35)為流動相,流速為1.0 mL·min-1,檢測波長為282 nm.濃度在0.025~5.000μg·mL-1範圍內多菌靈的質量濃度與峰麵積線性關繫良好,R2為0.9999;平均迴收率為83.60%~91.59%,RSD為2.11%~6.56%,方法的最低檢齣限(3S/N)為0.01 mg·kg-1.該方法操作簡單,靈敏度、重現性良好.
건립료일충분석고교중다균령잔류량적고효액상색보법( HPLC).고교분수후,용을산을지초성제취,정화、농축、용을정정용.채용배유자외검측기적HPLC측정,이을정-수(체적비위65∶35)위류동상,류속위1.0 mL·min-1,검측파장위282 nm.농도재0.025~5.000μg·mL-1범위내다균령적질량농도여봉면적선성관계량호,R2위0.9999;평균회수솔위83.60%~91.59%,RSD위2.11%~6.56%,방법적최저검출한(3S/N)위0.01 mg·kg-1.해방법조작간단,령민도、중현성량호.
The method was established to the detect the carbendazim residue in buckwheat by the high performance liquid chromatography( HPLC). The buckwheat samples were splintered,extracted with ethyl acetate by ultrasonic wave,purified,concentrated and diluted with acetonitrile. The experiments were performed by HPLC equipped with VWD. Experiment conditions are as follows:mobile phase,acetonitrile∶ water( 65∶ 35 );flow rate,1 mL · min1;wavelength,282 nm. The peak area was linearly proportional to carbendazim concentration in the range between 0. 025~5. 000 μg·mL-1 . The results showed the R2 was 0. 999 9,RSD was 2. 11% ~6. 56%,the average recov-ery was 83. 60% ~91. 59%,and the lowest detection limit was 0. 01 mg·kg-1 . The method has advantages of sim-ply operating,high sensitivity and good reproducibility.