CAJ | 학술논문

以丽江新团黑谷和谷梅二号为研究对象，利用稻瘟菌粗毒素分别对两者的幼苗根部处理0、6、12、24、48 h ，并进行地上部和地下部生理指标和相关基因表达分析研究。结果表明，毒素处理后，除SOD外，POD和CAT的活性均升高，MDA含量也升高。与水杨酸和茉莉酸途径相关的8个基因与本底水平相比都表现出上调或下调的趋势，可见这两个途径都得到了表达。特别是48 h时，PA L、J IOs PR10、PR1b和 PR4在稻瘟病抗性品种谷梅二号的根或叶中的表达量远高于敏感品种丽江新团黑谷，说明这4个基因的表达在调节谷梅二号抗稻瘟菌毒素中具有重要作用。
이려강신단흑곡화곡매이호위연구대상，이용도온균조독소분별대량자적유묘근부처리0、6、12、24、48 h ，병진행지상부화지하부생리지표화상관기인표체분석연구。결과표명，독소처리후，제SOD외，POD화CAT적활성균승고，MDA함량야승고。여수양산화말리산도경상관적8개기인여본저수평상비도표현출상조혹하조적추세，가견저량개도경도득도료표체。특별시48 h시，PA L、J IOs PR10、PR1b화 PR4재도온병항성품충곡매이호적근혹협중적표체량원고우민감품충려강신단흑곡，설명저4개기인적표체재조절곡매이호항도온균독소중구유중요작용。
In the present research ,Gumei 2 (resistant) and Lijang Xintuan Heigu (susceptible) are used to determine the physiological indices and related genes expression of the shoot and root of rice seedling under blast fungus toxin stress after 0 ,6 ,12 ,24 and 48 h .The results show that the activities of POD and CAT increase in addition to SOD ,and MDA content also increases after the toxin treatment .The 8 genes related with salicylic acid (SA) and jasmonic acid (JA) pathway show upward or downward trend compared with the background level , indicating that the two pathways have been expressed .Especially ,the expression levels of PAL ,JIOsPR10 ,PR1b and PR4 are higher in the resistant variety Gumei 2 than in the susceptible variety Xintuan Heigu at 48 h under the toxin treatment .The results suggest that the 4 genes play a considerable role in the regulation of Gumei 2 expressing resistance to blast fungus toxin stress .