商丘职业技术学院学报
商丘職業技術學院學報
상구직업기술학원학보
JOURNAL OF SHANGQIU VOCATIONAL AND TECHNICAL COLLEGE
2015年
2期
83-86
,共4页
虎掌南星%灭菌方法%培养基
虎掌南星%滅菌方法%培養基
호장남성%멸균방법%배양기
Rhizoma Pinelliae PedatisECTae%the sterilization method%medium
以虎掌南星（Rhizoma Pinelliae PedatisECTae），的块茎为试验材料，用不同消毒处理对虎掌南星外植体进行灭菌，并进行愈伤组织及丛生芽产生的初步研究．结果表明：虎掌南星外植体洗刷干净后使用75％乙醇溶液灭菌30s ，后再用01．％ HgCl2消毒15min ，每次消毒后用无菌水清洗5遍，每遍3min ．这种处理方法污染率较低．无论从平均愈伤组织诱导率还是从愈伤组织的质量上看，虎掌南星愈伤组织在M S上诱导优于B5．
以虎掌南星（Rhizoma Pinelliae PedatisECTae），的塊莖為試驗材料，用不同消毒處理對虎掌南星外植體進行滅菌，併進行愈傷組織及叢生芽產生的初步研究．結果錶明：虎掌南星外植體洗刷榦淨後使用75％乙醇溶液滅菌30s ，後再用01．％ HgCl2消毒15min ，每次消毒後用無菌水清洗5遍，每遍3min ．這種處理方法汙染率較低．無論從平均愈傷組織誘導率還是從愈傷組織的質量上看，虎掌南星愈傷組織在M S上誘導優于B5．
이호장남성（Rhizoma Pinelliae PedatisECTae），적괴경위시험재료，용불동소독처리대호장남성외식체진행멸균，병진행유상조직급총생아산생적초보연구．결과표명：호장남성외식체세쇄간정후사용75％을순용액멸균30s ，후재용01．％ HgCl2소독15min ，매차소독후용무균수청세5편，매편3min ．저충처리방법오염솔교저．무론종평균유상조직유도솔환시종유상조직적질량상간，호장남성유상조직재M S상유도우우B5．
Taking the tuber of tiger palm nanxing (Rhizoma Pinelliae PedatisECTae ,) as test materials ,with different disinfection treat‐ment on tiger palm south star explant for sterilization ,the preliminary study of the multiple shoot clumps is carried out .The experiment is that after washing clean sterilization 30s ,using 75% ethanol solution and then disinfected with 0 1.% HgCl2 ,the tiger palm south star explant is cleaned for 15 minutes ,every time washing 5 times with sterile water after disinfection ,every time 3 minutes .The results of this method of handling show that the pollution rate is low .From the power of the average callus induction rate of callus quality point of view ,tiger palm south star callus induced on MS is better than that of B5 .
