CAJ | 학술논문

目的：筛选50株白假丝酵母菌基因缺失菌，寻找出对白假丝酵母菌生物被膜形成相关基因，进一步探究白假丝酵母菌生物膜的致病机制。方法利用培养生物被膜的方法筛选50株基因缺失菌；利用 XTT 法验证所筛选出的白假丝酵母菌突变株 ORF19．2500生物被膜形成缺陷；进一步观察 ORF19．2500基因缺失菌生长、菌丝形成。结果用 XTT 法证明白假丝酵母菌突变株 orf19．2500生物膜形成缺陷，且生长曲线和滴琼脂平板的方法均提示其生长速率减慢。在 spider 培养基上白假丝酵母菌突变株 orf19．2500不能诱导菌丝形成，但在 YPD＋10％小牛血清则菌丝形成正常。结论白假丝酵母菌突变株 orf19．2500可利用 spider 培养基缺陷而影响其酵母、菌丝二态性的转化，及其生物被膜的形成。
목적：사선50주백가사효모균기인결실균，심조출대백가사효모균생물피막형성상관기인，진일보탐구백가사효모균생물막적치병궤제。방법이용배양생물피막적방법사선50주기인결실균；이용 XTT 법험증소사선출적백가사효모균돌변주 ORF19．2500생물피막형성결함；진일보관찰 ORF19．2500기인결실균생장、균사형성。결과용 XTT 법증명백가사효모균돌변주 orf19．2500생물막형성결함，차생장곡선화적경지평판적방법균제시기생장속솔감만。재 spider 배양기상백가사효모균돌변주 orf19．2500불능유도균사형성，단재 YPD＋10％소우혈청칙균사형성정상。결론백가사효모균돌변주 orf19．2500가이용 spider 배양기결함이영향기효모、균사이태성적전화，급기생물피막적형성。
Objective For searching some genes which impacted the formation of Candida albicans biofilm.Then we screened of 50 Candida albicans mutants.So as to identify potential pathogen mechanism of Candida albicans.Methods Screening 50 Candida albicans mutants in the method of cultivating biofilm in vivo.Biofilm was quantified by XTT.Then the mutants of growth rate and hy?phal formation were analyzed.Results It was defected for Candida albicans mutant orf19.2500 to form biofilm which was quantified by XTT.The mutant grows slowly,which was quantified by growth curve and spot assay.Then we find the mutant can not form hyphal in liquid spider media,while it can normally form hyphal in liquid YPD plus 10% fetal bovine serum media.Conclusion The Candi?da albicans mutant orf19.2500 can not utilize liquid media which impacted morphogenetic switching and the biofilm formation.