CAJ | 학술논문

目的比较近平滑念珠菌不同临床分离株的菌落和细胞形态及其分泌型天冬氨酸蛋白酶（SAP）酶活性，阐明该病原真菌的形态多样性及毒力差异。方法分别用 Lee’s GlcNAc、Lee’s glucose、Spider 3种培养基于37℃培养7株近平滑念珠菌临床分离株，观察各菌株的菌落及细胞形态；用牛血清白蛋白培养基（BSA）于30℃培养7株近平滑念珠菌，通过观察形成晕圈能力的强弱确定其毒力因子（SAP）的分泌活性。结果7株近平滑念珠菌临床分离株在不同条件下菌落表型有较明显的不同，菌落有光滑型、皱褶型及火山口样型，且染色程度不同，各分离株细胞在形成酵母相或菌丝相的能力方面呈现出不同程度的差异；SAP 酶活性观察实验中检测到各菌株水解晕圈大小从2．55 cm 到1．55 cm 各不相等。结论近平滑念珠菌临床分离株具有形态多样性，各菌株 SAP 酶活性明显不同，提示菌株间的毒力有明显差异。
목적비교근평활념주균불동림상분리주적균락화세포형태급기분비형천동안산단백매（SAP）매활성，천명해병원진균적형태다양성급독력차이。방법분별용 Lee’s GlcNAc、Lee’s glucose、Spider 3충배양기우37℃배양7주근평활념주균림상분리주，관찰각균주적균락급세포형태；용우혈청백단백배양기（BSA）우30℃배양7주근평활념주균，통과관찰형성훈권능력적강약학정기독력인자（SAP）적분비활성。결과7주근평활념주균림상분리주재불동조건하균락표형유교명현적불동，균락유광활형、추습형급화산구양형，차염색정도불동，각분리주세포재형성효모상혹균사상적능력방면정현출불동정도적차이；SAP 매활성관찰실험중검측도각균주수해훈권대소종2．55 cm 도1．55 cm 각불상등。결론근평활념주균림상분리주구유형태다양성，각균주 SAP 매활성명현불동，제시균주간적독력유명현차이。
Objective Compared the colonies and cells morphology and the secreted aspartic proteinase (SAP) enzyme activity of different clinical isolates of Candida parapsilosis,and elucidated the morphological diversity and the differences of virulence of the pathogenic fungi.Method Seven clinical isolates of Candida parapsilosis were cultured respectively on different solid media,and the morphology characteristics of colonies and cells were observed and recorded.Strains were also cultured on solid medium of bovine ser?um albumin (BSA) at 30 ℃ for determining the secretory activity of the virulence factors (SAP) by observing the proteolytic halos. Result Diverse colonies and phenotypes of the above strains were checked under several sorts of conditions—smooth,crepe and cra?ter in various shades of stained color,and the ability of filamentation were also different from each other among the tested strains.In addition,the sizes of proteolytic halos were various from 2.55 cm to 1.55 cm.Conclusion There are diverse phenotypes which can be shown for the clinical isolates of Candida parapsilosis,and the SAP enzyme activities of them are distinct,which may imply the signifi?cant virulent differences among the strains.