中国实验方剂学杂志
中國實驗方劑學雜誌
중국실험방제학잡지
CHINESE JOURNAL OF EXPERIMENTAL TRADITIONAL MEDICAL FORMULAE
2015年
11期
61-64
,共4页
张雯雯%刘绍光%丛晓东%蔡宝昌%钱建华%方小民%彭美仙
張雯雯%劉紹光%叢曉東%蔡寶昌%錢建華%方小民%彭美仙
장문문%류소광%총효동%채보창%전건화%방소민%팽미선
近红外光谱%五加皮酒%甘草酸%木香烃内酯%去氢木香内酯
近紅外光譜%五加皮酒%甘草痠%木香烴內酯%去氫木香內酯
근홍외광보%오가피주%감초산%목향경내지%거경목향내지
near infrared spectroscopy%Wujiapi liquor%glycyrrhizic acid%costunolide%dehydrocostuslactone
目的:建立五加皮酒提取液中特征性成分的近红外光谱快速检测方法.方法:采用HPLC测定样品中甘草酸、木香烃内酯、去氢木香内酯的含量,运用偏最小二乘法(PLS)建立各含量与近红外光谱(NIRS)之间的多元校正模型,并对未知样品进行定量预测.结果:校正模型对甘草酸、木香烃内酯、去氢木香内酯的交叉验证均方差(RMSECV)分别为0.023 9,0.021 8,0.012 6,相关系数(R2)分别为0.978 0,0.978 7,0.975 7,经外部验证,预测均方差(RMSEP)分别为0.023 7,0.018 8,0.010 7.结论:利用近红外光谱法建立的模型具有良好的预测效果,能满足五加皮酒提取液中特征性成分快速测定的要求.
目的:建立五加皮酒提取液中特徵性成分的近紅外光譜快速檢測方法.方法:採用HPLC測定樣品中甘草痠、木香烴內酯、去氫木香內酯的含量,運用偏最小二乘法(PLS)建立各含量與近紅外光譜(NIRS)之間的多元校正模型,併對未知樣品進行定量預測.結果:校正模型對甘草痠、木香烴內酯、去氫木香內酯的交扠驗證均方差(RMSECV)分彆為0.023 9,0.021 8,0.012 6,相關繫數(R2)分彆為0.978 0,0.978 7,0.975 7,經外部驗證,預測均方差(RMSEP)分彆為0.023 7,0.018 8,0.010 7.結論:利用近紅外光譜法建立的模型具有良好的預測效果,能滿足五加皮酒提取液中特徵性成分快速測定的要求.
목적:건립오가피주제취액중특정성성분적근홍외광보쾌속검측방법.방법:채용HPLC측정양품중감초산、목향경내지、거경목향내지적함량,운용편최소이승법(PLS)건립각함량여근홍외광보(NIRS)지간적다원교정모형,병대미지양품진행정량예측.결과:교정모형대감초산、목향경내지、거경목향내지적교차험증균방차(RMSECV)분별위0.023 9,0.021 8,0.012 6,상관계수(R2)분별위0.978 0,0.978 7,0.975 7,경외부험증,예측균방차(RMSEP)분별위0.023 7,0.018 8,0.010 7.결론:이용근홍외광보법건립적모형구유량호적예측효과,능만족오가피주제취액중특정성성분쾌속측정적요구.
