CAJ | 학술논문

以德国鸢尾（ Iris germanica L．）品种“金娃娃”当年新萌发幼芽的茎尖为外植体，进行了组织培养和快速繁殖技术的研究。结果表明：使用0．1％HgCl2对外植体消毒7 min的效果最佳；初代培养、继代培养、生根培养最适合的培养基分别是MS＋6－BA 1．5 mg／L＋IAA 0．2 mg／L、MS＋6－BA 2．0 mg／L＋IAA 0．5 mg／L、1／2MS＋6－BA 0．2 mg／L＋IAA15．mg／L；采用含4～5个芽的芽丛进行继代培养的增殖系数最高；试管苗移栽的最佳基质为珍珠岩＋草炭＋园土（1∶1∶1），移栽成活率达到91．6％。
이덕국연미（ Iris germanica L．）품충“금왜왜”당년신맹발유아적경첨위외식체，진행료조직배양화쾌속번식기술적연구。결과표명：사용0．1％HgCl2대외식체소독7 min적효과최가；초대배양、계대배양、생근배양최괄합적배양기분별시MS＋6－BA 1．5 mg／L＋IAA 0．2 mg／L、MS＋6－BA 2．0 mg／L＋IAA 0．5 mg／L、1／2MS＋6－BA 0．2 mg／L＋IAA15．mg／L；채용함4～5개아적아총진행계대배양적증식계수최고；시관묘이재적최가기질위진주암＋초탄＋완토（1∶1∶1），이재성활솔체도91．6％。
The stem tips of newly-germinated tender buds of the German iris ( Iris germanica L.) variety “Gold boy” were taken as explants, and the tissue culture and rapid propagation techniques of iris were studied.The results showed that disinfecting the explants with 0.1%HgCl2 for 7 min was the most effective.The optimal media for the primary culture, subculture and rooting culture of iris were MS+6-BA 1.5 mg/L+IAA 0.2 mg/L, MS+6-BA 2.0 mg/L+IAA 0.5 mg/L, and 1/2MS+6-BA 0.2 mg/L+IAA 1.5 mg/L, respectively.The subculture of bud cluster with 4~5 buds obtained the highest propagation coefficient.The optimum sub-strate for the transplanting of iris test-tube plantlets was perlite +turf+garden soil ( 1∶1∶1) , and the survival rate after being trans-planted reached 91.6%.