CAJ | 학술논문

目的：探讨氢气生理盐水（Hydrogen－rich saline， HS）对大鼠下肢缺血再灌注（Limb ischemia reperfusion， LIR）引起的急性肺损伤（Acute lung injury， ALI）的保护作用。方法成年雄性 SD 大鼠30只，随机均分为5组：生理盐水对照组（CON 组）、模型组（LIR 组）、氢气盐水（2．5 mL／kg、5 mL／kg、10 mL／kg）预处理组（HS1、HS2、HS3组）。LIR 组大鼠使用橡皮筋高位结扎双下肢3小时后恢复灌注3 h；氢气盐水处理组大鼠在结扎前30分钟腹腔注射不同剂量氢气盐水，其余两组分别静脉给予相同容量的生理盐水。恢复灌注3小时后取大鼠颈内动脉血进行血气分析，并进行一侧肺泡灌洗，ELSIA 法检测另一侧肺组织中 MDA 和 GSH 的浓度。检测收集的肺泡灌洗液中 TNF－α和IL－1β的浓度。结果 LIR 组病理学评分显著高于 CON 组而氧合指数低于 CON 组；HS2和 HS3组病理学评分低于LIR 组而氧合指数高于 LIR 组，均有统计学意义（P ＜0．01）。与 CON 组相比，LIR 组大鼠肺泡灌洗液中 TNF－α和IL－1β浓度、肺组织中 MDA 浓度显著升高；肺组织中 GSH 的浓度显著降低（均 P ＜0．01）。 HS1、HS2和 HS3组肺泡灌洗液中 TNF－α和 IL－1β浓度、肺组织中 GSH 浓度均显著高于 LIR 组，MDA 浓度低于 LIR 组（均 P ＜0．01）。结论HS 可减轻大鼠下肢 LIR 引起的 ALI，HS 的作用可能与其减轻炎症反应和氧化应激反应有关。
목적：탐토경기생리염수（Hydrogen－rich saline， HS）대대서하지결혈재관주（Limb ischemia reperfusion， LIR）인기적급성폐손상（Acute lung injury， ALI）적보호작용。방법성년웅성 SD 대서30지，수궤균분위5조：생리염수대조조（CON 조）、모형조（LIR 조）、경기염수（2．5 mL／kg、5 mL／kg、10 mL／kg）예처리조（HS1、HS2、HS3조）。LIR 조대서사용상피근고위결찰쌍하지3소시후회복관주3 h；경기염수처리조대서재결찰전30분종복강주사불동제량경기염수，기여량조분별정맥급여상동용량적생리염수。회복관주3소시후취대서경내동맥혈진행혈기분석，병진행일측폐포관세，ELSIA 법검측령일측폐조직중 MDA 화 GSH 적농도。검측수집적폐포관세액중 TNF－α화IL－1β적농도。결과 LIR 조병이학평분현저고우 CON 조이양합지수저우 CON 조；HS2화 HS3조병이학평분저우LIR 조이양합지수고우 LIR 조，균유통계학의의（P ＜0．01）。여 CON 조상비，LIR 조대서폐포관세액중 TNF－α화IL－1β농도、폐조직중 MDA 농도현저승고；폐조직중 GSH 적농도현저강저（균 P ＜0．01）。 HS1、HS2화 HS3조폐포관세액중 TNF－α화 IL－1β농도、폐조직중 GSH 농도균현저고우 LIR 조，MDA 농도저우 LIR 조（균 P ＜0．01）。결론HS 가감경대서하지 LIR 인기적 ALI，HS 적작용가능여기감경염증반응화양화응격반응유관。
Objective To evaluate the effect of hydrogen-rich saline (HS) in protecting acute lung injury induced by lower limb ischemia reperfusion in rats.Method 30 adult male SD rats were chosen as the subjects and were randomly divided into 5 groups: normal saline control group (group CON), model group (group LIR), hydrogen saline (2.5ml/kg, 5ml /kg, 10ml /kg) preconditioning group (HS1, HS2, HS3 groups).3-hour reperfusion was conducted for the model group rats after a rubber band ligation on the lower limbs had been executed for 3 hours.The rats treated with hydrogen saline 30 minutes before ligation were given intraperitoneal injection of hydrogen saline (2.5ml/kg, 5ml /kg, 10ml /kg).The normal saline group were given the same amount of saline as given to the other two groups.3-h after reperfusion, sample carotid artery blood was taken for blood gas analysis and conducted bronchoalveolar lavage on the side and then tested the concentration of MDA and GSH on the other side of lung by tissue pathology detection.Then detected the concentration of cell factors TNF-alpha and IL-1 beta as collected in the alveolar lavage fluid.Findings The LIR group’s pathological score was significantly higher than that of the CON group while its oxygenation index lower than that of the CON group (P<0.01).The pathological score of HS2 group and HS3 group was lower than that of the LIR group while the two groups’ oxygenation index was higher than that of the LIR group (P <0.01).Compared with the CON group, the LIR group’s concentration of MDA and the inflammation factors TNF-alpha and IL-1 beta in bronchoalveolar lavage fluid significantly increased (P <0.01); GSH concentration in the lung tissue, however, decreased significantly (P <0.01).HS1, HS2 and HS3 groups’concentration indexes of MDA and inflammation factors TNF-alpha and IL-1 beta in bronchoalveolar lavage fluid was significantly higher than those as observed in the LIR group (P <0.01).Concentration of GSH in the lung tissue was also higher than that of the LIR group (P <0.01).Conclusion HS can reduce ALI in the lower limbs caused by LIR.The effect of HS may be correlated with the inflammatory reaction and the oxidative stress reaction.