微生物学杂志
微生物學雜誌
미생물학잡지
JOURNAL OF MICROBIOLOGY
2015年
2期
103-108
,共6页
宫小明%马荣桧%孙军%张艺兵%于金玲%丁葵英%郭礼强
宮小明%馬榮檜%孫軍%張藝兵%于金玲%丁葵英%郭禮彊
궁소명%마영회%손군%장예병%우금령%정규영%곽례강
黄曲霉%寄生曲霉%黄曲霉毒素%芽胞杆菌%拮抗物%液质联用
黃麯黴%寄生麯黴%黃麯黴毒素%芽胞桿菌%拮抗物%液質聯用
황곡매%기생곡매%황곡매독소%아포간균%길항물%액질련용
Aspergillus flavus%Aspergillus parasiticus%aflatoxin%antifungal%Bacillus%LC-MS/ MS
筛选黄曲霉毒素生物防控菌,为黄曲霉毒素的生物防控提供支持。以花生原产地土壤为材料,采用牛津杯法筛选所需菌株。对筛选出的拮抗菌株进行抑制产毒曲霉菌株的生长、产孢、降解黄曲霉毒素实验。筛选出2株黄曲霉毒素生防细菌,编号21-1-2、17-3,经鉴定,拮抗菌21-1-2为枯草芽胞杆菌,拮抗菌17-3为地衣芽胞杆菌。分别对拮抗菌对曲霉孢子萌发的抑制、抑制黄曲霉的生长和菌丝延长以及减少黄曲霉毒素的产生、对黄曲霉毒素的分解作用等几个方面进行研究,结果表明,拮抗菌可以明显抑制产毒曲霉孢子的萌发、生长、菌丝的延长,减少黄曲霉毒素的产生以及分解黄曲霉毒素。
篩選黃麯黴毒素生物防控菌,為黃麯黴毒素的生物防控提供支持。以花生原產地土壤為材料,採用牛津杯法篩選所需菌株。對篩選齣的拮抗菌株進行抑製產毒麯黴菌株的生長、產孢、降解黃麯黴毒素實驗。篩選齣2株黃麯黴毒素生防細菌,編號21-1-2、17-3,經鑒定,拮抗菌21-1-2為枯草芽胞桿菌,拮抗菌17-3為地衣芽胞桿菌。分彆對拮抗菌對麯黴孢子萌髮的抑製、抑製黃麯黴的生長和菌絲延長以及減少黃麯黴毒素的產生、對黃麯黴毒素的分解作用等幾箇方麵進行研究,結果錶明,拮抗菌可以明顯抑製產毒麯黴孢子的萌髮、生長、菌絲的延長,減少黃麯黴毒素的產生以及分解黃麯黴毒素。
사선황곡매독소생물방공균,위황곡매독소적생물방공제공지지。이화생원산지토양위재료,채용우진배법사선소수균주。대사선출적길항균주진행억제산독곡매균주적생장、산포、강해황곡매독소실험。사선출2주황곡매독소생방세균,편호21-1-2、17-3,경감정,길항균21-1-2위고초아포간균,길항균17-3위지의아포간균。분별대길항균대곡매포자맹발적억제、억제황곡매적생장화균사연장이급감소황곡매독소적산생、대황곡매독소적분해작용등궤개방면진행연구,결과표명,길항균가이명현억제산독곡매포자적맹발、생장、균사적연장,감소황곡매독소적산생이급분해황곡매독소。
Aflatoxin bio-controlling bacterial strain was screened in order to provide support for controlling aflatoxin. The growth,sporogenesis,and aflatoxin degradable experiments were carried out to the needed and inhibition strains screened from soil collected in peanut source land adopting Oxford-cup method. Two aflatoxin bio-controlling bacterial strains were screened and numbered as 21-1-2 and 17-3,they were characterized by authorized institution,the inhibi-tion strain 21-1-2 to be Bacillus subtilis,and inhibition strain 17-3 to be Bacillus licheniformis. They were studied on several aspects including the inhibition against the germination of Aspergillus spores,the inhibition against the growth of A. flavus,the extension of their mycelia,and the reduction of the aflatoxin production,and the degradation effects of aflatoxin. The results showed that the inhibition strains could significantly inhibit the spore germination of toxin-pro-ducing Aspergillus strains,their growth,and the extension of their mycelia,reduce the production of aflatoxin,as well as the degradation of aflatoxin.