微生物学杂志
微生物學雜誌
미생물학잡지
JOURNAL OF MICROBIOLOGY
2015年
2期
95-97
,共3页
卡介苗(BCG)%THP-1%p300%乙酰化
卡介苗(BCG)%THP-1%p300%乙酰化
잡개묘(BCG)%THP-1%p300%을선화
BCG%THP-1%P300%acetylation
研究 p300乙酰化在卡介苗(bacillus Calmette-Guérin,BCG)感染中的作用。构建 THP-1巨噬细胞模型,比较 BCG 感染前后 p300蛋白表达水平和组蛋白 H3乙酰化水平的改变,加入 p300特异性抑制剂 Delphini-din,观察细胞内组蛋白 H3乙酰化水平的变化。结果表明,在分化成熟的 THP-1细胞系中,BCG 感染能够上调p300蛋白表达水平和组蛋白 H3乙酰化水平,加入 p300特异性抑制剂 Delphinidin 后,组蛋白 H3乙酰化水平降低。BCG 感染通过 p300途径导致蛋白质乙酰化水平发生改变。
研究 p300乙酰化在卡介苗(bacillus Calmette-Guérin,BCG)感染中的作用。構建 THP-1巨噬細胞模型,比較 BCG 感染前後 p300蛋白錶達水平和組蛋白 H3乙酰化水平的改變,加入 p300特異性抑製劑 Delphini-din,觀察細胞內組蛋白 H3乙酰化水平的變化。結果錶明,在分化成熟的 THP-1細胞繫中,BCG 感染能夠上調p300蛋白錶達水平和組蛋白 H3乙酰化水平,加入 p300特異性抑製劑 Delphinidin 後,組蛋白 H3乙酰化水平降低。BCG 感染通過 p300途徑導緻蛋白質乙酰化水平髮生改變。
연구 p300을선화재잡개묘(bacillus Calmette-Guérin,BCG)감염중적작용。구건 THP-1거서세포모형,비교 BCG 감염전후 p300단백표체수평화조단백 H3을선화수평적개변,가입 p300특이성억제제 Delphini-din,관찰세포내조단백 H3을선화수평적변화。결과표명,재분화성숙적 THP-1세포계중,BCG 감염능구상조p300단백표체수평화조단백 H3을선화수평,가입 p300특이성억제제 Delphinidin 후,조단백 H3을선화수평강저。BCG 감염통과 p300도경도치단백질을선화수평발생개변。
The role of p300 acetylation of BCG(bacillus,Calmette-Guérin)infection was studied. THP-1 macro-phage model was constructed to compare the changes of the expression level of p300 protein and histone H3 acetylation level before and after BCG infection,adding p300 specific inhibitor of delphinidin,and observe the changes of acety-lation level in H3 histone. The results showed that in a mature differentiation of THP-1 cell system,the BCG infection could up regulated the protein expression level of p300 and histone H3 acetylation level,and after adding p300 specif-ic inhibition preparation of delphinidin,the level of histone H3 acetylation reduced. Therefore,BCG infection may lead to the change of protein acetylation level through p300 pathways.