微生物学杂志
微生物學雜誌
미생물학잡지
JOURNAL OF MICROBIOLOGY
2015年
2期
90-94
,共5页
油脂降解%鉴定%克雷伯氏菌X-1%优化
油脂降解%鑒定%剋雷伯氏菌X-1%優化
유지강해%감정%극뢰백씨균X-1%우화
oil degradation%identification%Klebsiella sp. X-1%optimization
从学校食堂排放的废水中筛选出1株油脂降解菌株，经形态特征、生理生化特征、16S rDNA 同源性序列分析，鉴定为克雷伯氏菌属，暂命名为 Klebsiella sp. X-1。并利用正交实验进一步考察了 pH、培养温度、摇床转速对油脂降解率的影响。实验结果表明，在高含油废水培养基中，pH 7.0、转速为140 r/ min、培养温度为30℃时，72 h 内该菌的油脂降解率最高达68.2%。
從學校食堂排放的廢水中篩選齣1株油脂降解菌株，經形態特徵、生理生化特徵、16S rDNA 同源性序列分析，鑒定為剋雷伯氏菌屬，暫命名為 Klebsiella sp. X-1。併利用正交實驗進一步攷察瞭 pH、培養溫度、搖床轉速對油脂降解率的影響。實驗結果錶明，在高含油廢水培養基中，pH 7.0、轉速為140 r/ min、培養溫度為30℃時，72 h 內該菌的油脂降解率最高達68.2%。
종학교식당배방적폐수중사선출1주유지강해균주，경형태특정、생리생화특정、16S rDNA 동원성서렬분석，감정위극뢰백씨균속，잠명명위 Klebsiella sp. X-1。병이용정교실험진일보고찰료 pH、배양온도、요상전속대유지강해솔적영향。실험결과표명，재고함유폐수배양기중，pH 7.0、전속위140 r/ min、배양온도위30℃시，72 h 내해균적유지강해솔최고체68.2%。
An oil degradable bacterial strain was screened from wastewater discharged from the university dining hall. It was identified as Klebsiella sp. X-1 through its morphological,physiological and biochemical features,and 16S rDNA homology sequencing analysis. The effects of pH,temperature,and shaking rpm on oil degradation were further observed adopted with orthogonal experiment. The results showed that within 72 hours,in the high oily wastewater me-dium,the optimum process conditions were as follows:pH 7,shaking at 140 r/ min,cultured at 30 ℃,the oil de-gradable rate of the strain reached at the highest of 68. 2% .
