CAJ | 학술논문

脂肪酶可以催化甘油三酯水解成脂肪酸和甘油，已广泛应用在工业领域，而获得产酶微生物是研究的基础。采用油脂平板法筛选出1株脂肪酶产生菌。经16S rRNA 序列分析可知，该菌株属于柠檬酸杆菌（Citrobacter werkman and Gillen）。单因素试验对其进行产酶条件优化，优化后产酶条件（g/ L）：淀粉2.0，KH2 PO41.0，K2 HPO4·3H2 O 2.2，（NH4）2 SO41.0，MgSO4·7H2 O 0.1，牛肉膏2.0，橄榄油10.0 mL，pH 7.5，每升接种量为15 mL，37℃培养43 h。获得最大酶活为384 U/ mL，是优化前的13倍。可以利用该菌制备脂肪酶。
지방매가이최화감유삼지수해성지방산화감유，이엄범응용재공업영역，이획득산매미생물시연구적기출。채용유지평판법사선출1주지방매산생균。경16S rRNA 서렬분석가지，해균주속우저몽산간균（Citrobacter werkman and Gillen）。단인소시험대기진행산매조건우화，우화후산매조건（g/ L）：정분2.0，KH2 PO41.0，K2 HPO4·3H2 O 2.2，（NH4）2 SO41.0，MgSO4·7H2 O 0.1，우육고2.0，감람유10.0 mL，pH 7.5，매승접충량위15 mL，37℃배양43 h。획득최대매활위384 U/ mL，시우화전적13배。가이이용해균제비지방매。
Lipase can catalyze hydrolysis of triglyceride into fatty acids and glycerol,and has been widely applied in industries,and to obtain lipase-producing microorganisms is the basis of the study. A novel lipase-producing microbe was isolated from lipid-contaminated soil samples using plate-screening method. It was purified and identified as Citrobacter sp. through 16S rRNA sequence analysis. The enzyme production condition was optimized by single factor experiment. The optimum culture condition in a liter medium was 2. 0(g/ L,the same below)of soluble starch,1. 0 KH2 PO4 ,2. 2 K2 HPO4 ·3H2 O,1. 0(NH4 )2 SO4 ,0. 1 MgSO4 ·7H2 O,2. 0 beef extract,olive oil 10. 0 mL,pH 7. 5 and 15 mL of inoculum,culture for 43 h at 37 °C in shaker. The obtained enzymatic activity was 384 U/ mL at the highest,and it was 13-fold of that before the optimization. This strain could be used to produce lipase.