CAJ | 학술논문

目的：验证牙髓暴露法建立大鼠实验性牙髓炎痛敏模型的可行性。方法：取健康雄性SD大鼠36只，随机分为2组；对照组（n＝10）麻醉后不作任何处理，牙髓炎模型组（n＝26）麻醉后于左侧上颌第一磨牙穿髓并使之暴露于口腔。然后，每组中各随机抽取6只分别检测其术前及术后1、3、7、14 d的擦面行为。对照组所余4只于术后2 h取延髓及牙髓，牙髓炎组所余20只分别于术后2 h 取延髓，1、3、7、14 d取牙髓；用免疫荧光染色检测延髓中c－Fos蛋白及牙髓中TNF－α的表达，HE染色观察牙髓炎症情况。结果：①牙髓炎组在建模术后1d时的擦面总时间最高，其次为术后3d组，两者分别与组内其他各时间相比，以及与同一时间点的对照组相比差异均有统计学意义（P＜0．05）；②HE染色显示，牙髓暴露1 d时即可见明显炎症，术后1、3、7、14 d，其坏死组织的边缘分别位于髓角和根上、中、下1／3处；③牙髓炎组在术后1、3、7、14 d各时间点的TNF－α表达强度的平均光密度值均明显高于对照组（P＜0．05）；④术后2 h牙髓炎组和对照组延髓背角的c－Fos 阳性细胞数为176．8±14．82和18．2±4．08（P＜0．05）。结论：牙髓暴露法能有效模拟临床牙髓炎及其痛敏过程。
목적：험증아수폭로법건립대서실험성아수염통민모형적가행성。방법：취건강웅성SD대서36지，수궤분위2조；대조조（n＝10）마취후불작임하처리，아수염모형조（n＝26）마취후우좌측상합제일마아천수병사지폭로우구강。연후，매조중각수궤추취6지분별검측기술전급술후1、3、7、14 d적찰면행위。대조조소여4지우술후2 h취연수급아수，아수염조소여20지분별우술후2 h 취연수，1、3、7、14 d취아수；용면역형광염색검측연수중c－Fos단백급아수중TNF－α적표체，HE염색관찰아수염증정황。결과：①아수염조재건모술후1d시적찰면총시간최고，기차위술후3d조，량자분별여조내기타각시간상비，이급여동일시간점적대조조상비차이균유통계학의의（P＜0．05）；②HE염색현시，아수폭로1 d시즉가견명현염증，술후1、3、7、14 d，기배사조직적변연분별위우수각화근상、중、하1／3처；③아수염조재술후1、3、7、14 d각시간점적TNF－α표체강도적평균광밀도치균명현고우대조조（P＜0．05）；④술후2 h아수염조화대조조연수배각적c－Fos 양성세포수위176．8±14．82화18．2±4．08（P＜0．05）。결론：아수폭로법능유효모의림상아수염급기통민과정。
AIM:To establish and verify rat experimental hyperalgesic pulpitis.METHODS:36 male SD rats were randomly divided into 2 groups.The rats in control group (n=10)received no treatment after anesthesia,while the left maxillary first molar pulp of the rats in the pulpitis group (n=26)were exposed after anesthesia.6 rats of each group were randomly selected for face grooming test before and after 1,3,7 and 14 d of surgery respectively.The medul-la oblongatas and pulps of the remaining 4 rats in the control group were taken after 2 h of surgery.The remaining 20 rats in the pulpitis group were randomly divided into 5 groups (n=4),the pulps were taken after 1,3,7 and 14 d of surger-y respectively.HE staining,TNF-αand c-Fos immunofluorescence staining were conducted to measure the changes of pulp and bulbus medullae.RESULTS:The face Grooming after 1 d or 3 d of surgery in pulpitis groups were longer than that in the control group (P<0.05 ).In the pulpitis groups longest face grooming was observed 1 d followed by 3 d (P<0.05)after surgery.HE staining revealed that 1,3,7 and 14 d after surgery,inflammation and necrotic tissues ap-peared at the pulp horn,cervical-third,middle-third and apical-third of the root respectively.The mean optical density of TNF-αat various time points of pulpitis group were significantly higher than that of control group(P<0.05).In pul-pitis group highest TNF-αlevel was observed 1d followed by 3 and 7d after surgery(P<0.05),the lowest at 14d after surgery(P<0.05).c-Fos immunostaining results showed that c-Fos-positive cells were significantly increased in medul-lary dorsal horn after 2 h of pulp exposure.CONCLUON:Pulp exposure method can effectively establish rat experimen-tal hyperalgesic pulpitis madel,which simulates the process of clinical pulpitis and hyperalgesia.