CAJ | 학술논문

目的：体外观察他莫昔芬（Tamoxifen）对牙髓干细胞分化的作用。方法：培养人牙髓干细胞（hDPSCs），将hDPSCs用不同浓度的他莫昔芬刺激不同时间，CCK－8法检测细胞的增殖情况；矿化诱导培养2周后茜素红染色观察矿化结节的形成，RT－PCR检测各组矿化相关基因碱性磷酸酶（ALP）、骨钙素（OCN）、骨唾液酸蛋白（BSP）、牙本质涎磷蛋白（DSPP）的表达水平。结果：10μmol／L 他莫昔芬抑制hDPSCs增殖，其他浓度对hDPSCs无明显影响；与对照组相比0．5、1、2．5、5、10μmol／L他莫昔芬均能促进矿化结节的形成，其中5μmol／L 最明显；且与对照组相比，5μmol／L他莫昔芬明显上调矿化相关基因ALP、OCN、BSP 和DSPP 的mR-NA表达水平。结论：他莫昔芬可明显促进牙髓干细胞向成骨／成牙分化。
목적：체외관찰타막석분（Tamoxifen）대아수간세포분화적작용。방법：배양인아수간세포（hDPSCs），장hDPSCs용불동농도적타막석분자격불동시간，CCK－8법검측세포적증식정황；광화유도배양2주후천소홍염색관찰광화결절적형성，RT－PCR검측각조광화상관기인감성린산매（ALP）、골개소（OCN）、골타액산단백（BSP）、아본질연린단백（DSPP）적표체수평。결과：10μmol／L 타막석분억제hDPSCs증식，기타농도대hDPSCs무명현영향；여대조조상비0．5、1、2．5、5、10μmol／L타막석분균능촉진광화결절적형성，기중5μmol／L 최명현；차여대조조상비，5μmol／L타막석분명현상조광화상관기인ALP、OCN、BSP 화DSPP 적mR-NA표체수평。결론：타막석분가명현촉진아수간세포향성골／성아분화。
AIM:To investigate the effects of tamoxifen on the osteoblastic/odontoblastic differentiation of human dental pulp stem cells (hDPSCs)in vitro.METHODS:hDPSCs were treated by different concentrations of tamoxifen.Kit-8 assay was applied to assess cell proliferation.After mineralization induction culture for 2 weeks,aliz-arin red S staining was used to observe the mineral nodules,the mRNA expression of the alkaline phosphatase (ALP ),osteonectin (OCN),bone sialoprotein (BSP)and dentin sialophosphoprotein (DSPP)was detected by RT-PCR. RESULITS:Tamoxifen at 10μmol/L inhibited hDPSCs proliferation and lower concentration showed no effect.All concentration of tamoxifen increased mineral nodule formation of hDPSCs,5 μmol/L tamoxifen showed the most signifi-cant effect(P<0.05).Furthermore,the mRNA expression of ALP,OCN,BSP and DSPP was markedly upregulated by 5 μmol/L tamoxifen treatment.CONCLUSION:Tamoxifen promotes mineralization and mineralization-related genes expression and might play a vital role on the differentiation of hDPSCs.