微生物学杂志
微生物學雜誌
미생물학잡지
JOURNAL OF MICROBIOLOGY
2015年
2期
78-84
,共7页
茶树%内生真菌%漆酶%培养基成分%发酵条件
茶樹%內生真菌%漆酶%培養基成分%髮酵條件
다수%내생진균%칠매%배양기성분%발효조건
Camellia sinensis%endophytic fungi%laccase%medium components%fermentation condition
从茶树内生真菌筛选产漆酶的菌株，分析不同营养因素和培养条件对菌株漆酶酶活力的影响。采用6种显色底物的平板初筛和酶活测定的复筛方法，从15株茶树内生真菌菌株中筛选获得1株产漆酶酶活较高的菌株 CSN-4。单因素分析结果显示，液态发酵条件下菌株 CSN-4适宜的主要培养基成分是麸皮和蛋白胨；菌株 CSN-4分别在麸皮30 g / L、蛋白胨2.5 g / L、CuSO4·5H2 O 0.015 g / L 和茶水6 g / L 时发酵产漆酶酶活最高。发酵条件试验结果表明，菌株 CSN-4分别在接种量为6个菌饼（直径6 mm）、装液量60 mL /250 mL、pH 4.8、摇床转速120 r / min，培养温度为28℃时产漆酶酶活较高。在培养基中添加麸皮和茶水对菌株 CSN-4产漆酶有明显的促进作用。经过培养基成分及培养条件优化后，菌株 CSN-4产漆酶酶活显著升高，达到2417 U / L。
從茶樹內生真菌篩選產漆酶的菌株，分析不同營養因素和培養條件對菌株漆酶酶活力的影響。採用6種顯色底物的平闆初篩和酶活測定的複篩方法，從15株茶樹內生真菌菌株中篩選穫得1株產漆酶酶活較高的菌株 CSN-4。單因素分析結果顯示，液態髮酵條件下菌株 CSN-4適宜的主要培養基成分是麩皮和蛋白胨；菌株 CSN-4分彆在麩皮30 g / L、蛋白胨2.5 g / L、CuSO4·5H2 O 0.015 g / L 和茶水6 g / L 時髮酵產漆酶酶活最高。髮酵條件試驗結果錶明，菌株 CSN-4分彆在接種量為6箇菌餅（直徑6 mm）、裝液量60 mL /250 mL、pH 4.8、搖床轉速120 r / min，培養溫度為28℃時產漆酶酶活較高。在培養基中添加麩皮和茶水對菌株 CSN-4產漆酶有明顯的促進作用。經過培養基成分及培養條件優化後，菌株 CSN-4產漆酶酶活顯著升高，達到2417 U / L。
종다수내생진균사선산칠매적균주，분석불동영양인소화배양조건대균주칠매매활력적영향。채용6충현색저물적평판초사화매활측정적복사방법，종15주다수내생진균균주중사선획득1주산칠매매활교고적균주 CSN-4。단인소분석결과현시，액태발효조건하균주 CSN-4괄의적주요배양기성분시부피화단백동；균주 CSN-4분별재부피30 g / L、단백동2.5 g / L、CuSO4·5H2 O 0.015 g / L 화다수6 g / L 시발효산칠매매활최고。발효조건시험결과표명，균주 CSN-4분별재접충량위6개균병（직경6 mm）、장액량60 mL /250 mL、pH 4.8、요상전속120 r / min，배양온도위28℃시산칠매매활교고。재배양기중첨가부피화다수대균주 CSN-4산칠매유명현적촉진작용。경과배양기성분급배양조건우화후，균주 CSN-4산칠매매활현저승고，체도2417 U / L。
A laccase-producing fungal strain CSN-4 was screened from 15 endophytic fungi isolated from healthy tea (Camellia sinensis)leaves with PDA medium plate amended with six coloration substrates for initial screening and re-screening by checking their laccase activity,and the effects of different nutritional factors and cultural condition on laccase activity of the strain were analyzed. The results of single factor analysis showed that the main medium compo-nents under liquid fermentation suitable for strain were wheat bran and peptone;laccase activity of strain CSN-4 reached the peak when fermented with 30 g/ L of wheat bran,2. 5 g/ L of peptone,0. 015 g/ L of CuSO4 ·5H2 O and 6 g/ L of tea infusion,respectively. The results of the experiments on fermentation conditions showed that when the fermentation was inoculated with six strain agar plugs(6 mm in diameter),liquid filling amount at 60 mL/ 250 mL, pH 4. 8,shaking at 120 r/ min,and culture temperature at 28 ℃,the laccase activity produced by the strain reached a fairly high. The addition of wheat bran and tea infusion had a significant improvement on laccase production by CSN-4. The laccase activity of CSN-4 significantly ascended and reached as high as 2 417 U/ L after the optimization of cul-ture medium compositions and culture conditions.
