CAJ | 학술논문

为了获取作为除臭微生物制剂的候选菌株，从垃圾渗滤液中分离筛选了4株具有对硫化氢和氨气高效降解菌株，分别标记为 CC3、CC7、CC13和 CC16。通过形态、生理生化和16S rDNA 序列分析，分别鉴定为乳酸片球菌（Pediococcus acidilactici）、巨大芽胞杆菌（Bacillus megaterium）、嗜酸乳杆菌（Lactobacillus acidophilus）、粪产碱杆菌（Alcaligenes faecalis），并确定复配菌剂的除臭能力。实验结果表明：菌株 CC7、CC13和 CC16组成的复配组合除臭效率最优，其最佳复配比例为1：1.5：0.5，接种量为5%，培养温度为30℃，初始培养基 pH值为6.5，培养时间为60 h 时，对氨气和硫化氢的去除率为最大值，可达到83.56%和70.25%。
위료획취작위제취미생물제제적후선균주，종랄급삼려액중분리사선료4주구유대류화경화안기고효강해균주，분별표기위 CC3、CC7、CC13화 CC16。통과형태、생리생화화16S rDNA 서렬분석，분별감정위유산편구균（Pediococcus acidilactici）、거대아포간균（Bacillus megaterium）、기산유간균（Lactobacillus acidophilus）、분산감간균（Alcaligenes faecalis），병학정복배균제적제취능력。실험결과표명：균주 CC7、CC13화 CC16조성적복배조합제취효솔최우，기최가복배비례위1：1.5：0.5，접충량위5%，배양온도위30℃，초시배양기 pH치위6.5，배양시간위60 h 시，대안기화류화경적거제솔위최대치，가체도83.56%화70.25%。
In order to obtain deodorant candidate strains of microbial preparation,four efficient HS and NH3 degrad-able strains were isolated from landfill leachate,and labeled as CC3,CC7,CC13 and CC16. They were identified as Pediococcus acidilactici,Bacillus megaterium,Lactobacillus acidophilus,and Alcaligenes faecalis respectively accord-ing to their morphology,physiological and biochemical test,and 16S rDNA sequence analysis,and confirmed the ca-pacity of the strain combination preparation in deodorizing efficacy. The experiment results showed that the deodorant effect was excellent when combined strains CC7,CC13 and CC16 together at the proportion of 1:1. 5:0. 5,with in-oculum at 5% ,initialize the medium pH at 6. 5,under 30 ℃ and cultivated for 60 hours,the removal rate of HS and NH3 reached to the maximum value as high as 83. 56% and 70. 25% .