牙体牙髓牙周病学杂志
牙體牙髓牙週病學雜誌
아체아수아주병학잡지
CHINESE JOURNAL OF CONSERVATIVE DENTISTRY
2015年
5期
277-281,262
,共6页
马逢乐%刘宝刚%王娟%何欣遥%王志华%何文喜
馬逢樂%劉寶剛%王娟%何訢遙%王誌華%何文喜
마봉악%류보강%왕연%하흔요%왕지화%하문희
人牙髓干细胞(hDPSCs)%ERK信号通路%增殖%分化
人牙髓榦細胞(hDPSCs)%ERK信號通路%增殖%分化
인아수간세포(hDPSCs)%ERK신호통로%증식%분화
human dental pulp stem cells(hDPSCs)%ERK signaling pathway%proliferation%differentiation
目的:探讨细胞外信号调节激酶(ERK)通路是否参与对人牙髓干细胞(hDPSCs)增殖及分化过程的调控。方法:用不同浓度的ERK通路抑制剂U0126干预hDPSCs,用CCK-8法检测细胞增殖;在矿化液诱导条件下,用不同浓度U0126干预hDPSCs 14 d,茜素红染色观察矿化结节的形成,RT-PCR检测成骨/成牙相关基因ALP、OCN、DSPP及BSP的表达;Western blot检测U0126(25μmol/L)干预后,ERK通路活性的变化。结果:不同浓度的U0126对hDPSCs的增殖能力无显著影响(P>0.05);与对照组相比,不同浓度的U0126对矿化结节的形成和成骨/成牙相关基因ALP、OCN、DSPP及BSP的表达均有抑制作用,25μmol/L U0126的抑制作用最明显(P<0.05);Western blot结果显示随着时间的延长,p-ERK的表达量逐步增加,在加入25μmol/L U0126后,p-ERK表达量下降(P<0.05)。结论:ERK信号通路可能不参与对hDPSCs增殖的调控,而在促进hDPSCs成骨/成牙分化过程中起调控作用。
目的:探討細胞外信號調節激酶(ERK)通路是否參與對人牙髓榦細胞(hDPSCs)增殖及分化過程的調控。方法:用不同濃度的ERK通路抑製劑U0126榦預hDPSCs,用CCK-8法檢測細胞增殖;在礦化液誘導條件下,用不同濃度U0126榦預hDPSCs 14 d,茜素紅染色觀察礦化結節的形成,RT-PCR檢測成骨/成牙相關基因ALP、OCN、DSPP及BSP的錶達;Western blot檢測U0126(25μmol/L)榦預後,ERK通路活性的變化。結果:不同濃度的U0126對hDPSCs的增殖能力無顯著影響(P>0.05);與對照組相比,不同濃度的U0126對礦化結節的形成和成骨/成牙相關基因ALP、OCN、DSPP及BSP的錶達均有抑製作用,25μmol/L U0126的抑製作用最明顯(P<0.05);Western blot結果顯示隨著時間的延長,p-ERK的錶達量逐步增加,在加入25μmol/L U0126後,p-ERK錶達量下降(P<0.05)。結論:ERK信號通路可能不參與對hDPSCs增殖的調控,而在促進hDPSCs成骨/成牙分化過程中起調控作用。
목적:탐토세포외신호조절격매(ERK)통로시부삼여대인아수간세포(hDPSCs)증식급분화과정적조공。방법:용불동농도적ERK통로억제제U0126간예hDPSCs,용CCK-8법검측세포증식;재광화액유도조건하,용불동농도U0126간예hDPSCs 14 d,천소홍염색관찰광화결절적형성,RT-PCR검측성골/성아상관기인ALP、OCN、DSPP급BSP적표체;Western blot검측U0126(25μmol/L)간예후,ERK통로활성적변화。결과:불동농도적U0126대hDPSCs적증식능력무현저영향(P>0.05);여대조조상비,불동농도적U0126대광화결절적형성화성골/성아상관기인ALP、OCN、DSPP급BSP적표체균유억제작용,25μmol/L U0126적억제작용최명현(P<0.05);Western blot결과현시수착시간적연장,p-ERK적표체량축보증가,재가입25μmol/L U0126후,p-ERK표체량하강(P<0.05)。결론:ERK신호통로가능불삼여대hDPSCs증식적조공,이재촉진hDPSCs성골/성아분화과정중기조공작용。
AIM:To investigate the function of ERK signaling pathway in the regulation of proliferation and differentiation of human dental pulp stem cells (hDPSCs).METHODS:hDPSCs were treated with different concen-trations of the ERK signaling pathway inhibitor U0126,and the proliferation was measured by CCK-8 method.The mRNA expression of ALP,OCN,DSPP and BSP was determined by RT-PCR.Alizarin red staining was used to detect the mineralized nodules.ERK signaling pathway activation was analyzed by Western blot.RESULTS:ERK inhibitor U0126 had no significant influence on the proliferation of hDPSCs.U0126 inhibited osteogenic differentiation and denti-nogenic differentiation as observed by Alizarin red staining.U0126,especially at 25 μmol/L,down-regulated the ex-pression of ALP,OCN,DSPP and BSP.U0126 at 25 μmol/L significantly inhibited ERK signaling pathway activation. CONCLUSION:ERK signaling pathway is probably not involved in the proliferation of hDPSCs,but plays an impor-tant role in the osteogenic and dentinogenic differentiation of hDPSCs.