CAJ | 학술논문

以8个敏感菌株作为指示菌，采用管碟法对分离自湛江硇洲岛（20°52′N ~20°56′N，110°33′E ~110°38′E）潮汐带香港巨牡蛎（Crassostrea hongkongensis）中的72株细菌的发酵液进行抗菌筛选，并对阳性菌株进行基因组 DNA 提取、16S rRNA 基因 PCR 扩增和序列测定，继而进行系统发育分析。抗菌实验结果表明，受试菌株中有23株菌的发酵产物具有抗菌活性（阳性率31.9%），其中有5个菌株（JSM 111024、JSM 111027、JSM 111029、JSM 111076、JSM 111083）具有较强的抗菌活性。基于16S rRNA 基因序列的系统发育分析表明，这23株菌具有较高的类群多样性和物种多样性，属于3个系统发育群/门（Alpha-Proteobacteria、Gamma-Proteobacte-ria、Bacteroidetes）中的8个科（Aeromonadaceae、Flavobacteriaceae、Halomonadaceae、Idiomarinaceae、Phyllobacteri-aceae、Pseudoalteromonadaceae、Shewanellaceae、Xanthomonadaceae）的8个属（Idiomarina、Halomonas、Myroides、Ni-tratireductor、Oceanimonas、Pseudoalteromonas、Shewanella、Wohlfahrtiimonas），可分为11个物种。优势类群为Gamma-Proteobacteria 亚门（14株），其中优势属为 Oceanimonas 属（6株）；第二大类群为 Bacteroidetes 门（7株），都属于 Flavobacteriaceae 科的 Myroides 属。具有较强抗菌活性的5个菌株中，有4个菌株（JSM 111024、JSM 111027、JSM 111029、JSM 111083）属于 Alpha-Proteobacteria 亚门 Phyllobacteriaceae 科 Oceanimonas 属，而菌株 JSM 111076属于 Gamma-Proteobacteria 亚门 Aeromonadaceae 科 Nitratireductor 属。
이8개민감균주작위지시균，채용관설법대분리자담강뇨주도（20°52′N ~20°56′N，110°33′E ~110°38′E）조석대향항거모려（Crassostrea hongkongensis）중적72주세균적발효액진행항균사선，병대양성균주진행기인조 DNA 제취、16S rRNA 기인 PCR 확증화서렬측정，계이진행계통발육분석。항균실험결과표명，수시균주중유23주균적발효산물구유항균활성（양성솔31.9%），기중유5개균주（JSM 111024、JSM 111027、JSM 111029、JSM 111076、JSM 111083）구유교강적항균활성。기우16S rRNA 기인서렬적계통발육분석표명，저23주균구유교고적류군다양성화물충다양성，속우3개계통발육군/문（Alpha-Proteobacteria、Gamma-Proteobacte-ria、Bacteroidetes）중적8개과（Aeromonadaceae、Flavobacteriaceae、Halomonadaceae、Idiomarinaceae、Phyllobacteri-aceae、Pseudoalteromonadaceae、Shewanellaceae、Xanthomonadaceae）적8개속（Idiomarina、Halomonas、Myroides、Ni-tratireductor、Oceanimonas、Pseudoalteromonas、Shewanella、Wohlfahrtiimonas），가분위11개물충。우세류군위Gamma-Proteobacteria 아문（14주），기중우세속위 Oceanimonas 속（6주）；제이대류군위 Bacteroidetes 문（7주），도속우 Flavobacteriaceae 과적 Myroides 속。구유교강항균활성적5개균주중，유4개균주（JSM 111024、JSM 111027、JSM 111029、JSM 111083）속우 Alpha-Proteobacteria 아문 Phyllobacteriaceae 과 Oceanimonas 속，이균주 JSM 111076속우 Gamma-Proteobacteria 아문 Aeromonadaceae 과 Nitratireductor 속。
The antibiosis screening of the fermentation broths of 72 marine bacteria,isolated from Hongkong giant oyster(Crassostrea hongkongensis)collected the tidal flat in Naozhou Island of Zhanjiang city(20°52′N ~ 20°56′N, 110°33′E ~ 110°38′E)Guangdong Province,was carried out using cylinder-plate method with 8 sensitive strains as indicators(3 Gram-positive and 3 Gram-negative bacteria,and 2 fungi),and extract DNA from positive strains’ge-nome,carried out 16S rRNA genes PCR amplification and sequencing,and then systematic phylogenetic analysis. The results of antibiotic experiments showed that among them fermentation broth of 23 tested strains possessed antibiotic ac-tivity( positive rate at 31. 9% ), of which 5 strains ( JSM111024, JSM111027, JSM111029, JSM111076, JSM111083)exhibited strong antibiotic activity. Based on 16S rRNA sequence and phylogenetic analysis suggested the 23 strains possessed group diversity and species diversity,they belonged to 8 genera(Idiomarina,Halomonas, Wohlfahrtiimonas,Pseudoalteromonas,Shewanella,Oceanimonas,Nitratireductor,Myroides)of 8 families( Aero-monadaceae,Flavobacteriaceae,Halomonadaceae,Idiomarinaceae,Phyllobacteriaceae,Pseudoalteromonadaceae, Shewanellaceae,Xanthomonadaceae)in 3 phylogenetic groups/ phyla(Alpha-Proteobacteria,Gamma-Proteobacteria, Bacteroidetes),and divided into 11 species. And the dominant groups were Gamma-Proteobacteria subphylum(14 strains),among them the dominant genus was Oceanimonas(6 strains),and the second large groups was the phylum of Bacteroidetes(7 strains),all belonged to the genus of Myroides of the family of Flacobacteriaceae. There were four strains(JSM 111024,JSM 111027,JSM 111029,JSM 111083)among 5 strains possessing strong antibiotic activity, belonged to the genus Oceanimonas of the family of Phyllobacteriaceae,subphylum of Alpha-Proteobacteria,and strain JSM 111076 belonged to the genus Nitratireductor of the family of Aeromonadaceae,subphylum of Gamma-Proteobac-teria.