CAJ | 학술논문

目的：研究自发性老年性骨质疏松模型SAMP6小鼠骨髓间充质干细胞（BMMSCs）的衰老状态、分化功能及线粒体呼吸链复合体活性。方法：分离培养SAMP6及其对照品系SAMR1小鼠BMMSCs，分别以实时定量PCR（RT－PCR）、Western Blot及细胞染色的方法，检测衰老、分化相关指标，并分别检测线粒体呼吸链复合体活性及复合体相关基因的表达。结果：与对照品系SAMR1小鼠相比，SAMP6小鼠BMMSCs的衰老相关基因、蛋白水平及β－gal染色阳性率显著升高（P＜0．05）；成骨分化相关基因、蛋白水平及茜素红染色阳性率显著降低（P＜0．05）；线粒体呼吸链复合体Ⅰ和Ⅲ活性显著降低，且呼吸链复合体相关基因表达亦降低（P＜0．05）。结论：SAMP6小鼠BMMSCs存在细胞衰老及成骨分化功能障碍，其线粒体呼吸链复合体功能显著下降。
목적：연구자발성노년성골질소송모형SAMP6소서골수간충질간세포（BMMSCs）적쇠로상태、분화공능급선립체호흡련복합체활성。방법：분리배양SAMP6급기대조품계SAMR1소서BMMSCs，분별이실시정량PCR（RT－PCR）、Western Blot급세포염색적방법，검측쇠로、분화상관지표，병분별검측선립체호흡련복합체활성급복합체상관기인적표체。결과：여대조품계SAMR1소서상비，SAMP6소서BMMSCs적쇠로상관기인、단백수평급β－gal염색양성솔현저승고（P＜0．05）；성골분화상관기인、단백수평급천소홍염색양성솔현저강저（P＜0．05）；선립체호흡련복합체Ⅰ화Ⅲ활성현저강저，차호흡련복합체상관기인표체역강저（P＜0．05）。결론：SAMP6소서BMMSCs존재세포쇠로급성골분화공능장애，기선립체호흡련복합체공능현저하강。
AIM:To detect senescence condition,osteogenic differentiation ability and the activity of mito-chondrial respiration chain complex of bone marrow mesenchymal stem cells (BMMSCs)from senescence accelerated mouse strain prone 6 (SAMP6).METHODS:BMMSCs of SAMP6 and its control strain SAMR1 mice were isolated and cultured.The cell senescence and osteogenic differentiation ability were detected by RT-PCR,Western Blot and cell staining respectively.The function of mitochondrial respiration complex was examined by activity assay and related gene expression assay.RESULTS:Compared with control strain SAMR1 ,BMMSCs of SAMP6 BMMSCs of SAMP6 performed increase of cellular senescence ce related genes and protein level,increase ofβ-gal positive cell ratio(P<0.05),and decline of osteogenic function(P<0.05),.CONCLUSION:BMMSCs from SAMP6 perform senescence and osteogenic differentiation dysfunction with significant decline of mitochondrial respiration chain complex.