CAJ | 학술논문

基于过氧化氢酶（HRP）催化和硼酸特异性识别，成功制备了一种多壁碳纳米管－ HRP －聚氨基苯硼酸（MWCNTs － HRP － PABA）复合膜并用于过氧化氢生物传感器.紫外－可见和红外光谱表明 MWCNTs － HRP － PABA 复合物具有良好的生物相容性并保持了 HRP 的生物活性.电化学阻抗谱（EIS）和扫描电化学显微镜（SEM）表明，在HRP 酶的催化下，聚氨基苯硼酸成功聚合.在0.1 M PBS 溶液中，PABA 具有一对可逆的氧化还原峰，表明了 HRP 酶的活性中心与电极之间的直接电化学.该生物传感器性能良好，过氧化氢的浓度在1.21 mM 至20 M 之间具有良好的线性关系，其相关系数为0.9973，检测下限为5.0 M （S∕N ＝3）.因此，这种基于 HRP 催化和硼酸特异性识别的方法为设计独特的生物传感器提供了一个良好的平台.
기우과양화경매（HRP）최화화붕산특이성식별，성공제비료일충다벽탄납미관－ HRP －취안기분붕산（MWCNTs － HRP － PABA）복합막병용우과양화경생물전감기.자외－가견화홍외광보표명 MWCNTs － HRP － PABA 복합물구유량호적생물상용성병보지료 HRP 적생물활성.전화학조항보（EIS）화소묘전화학현미경（SEM）표명，재HRP 매적최화하，취안기분붕산성공취합.재0.1 M PBS 용액중，PABA 구유일대가역적양화환원봉，표명료 HRP 매적활성중심여전겁지간적직접전화학.해생물전감기성능량호，과양화경적농도재1.21 mM 지20 M 지간구유량호적선성관계，기상관계수위0.9973，검측하한위5.0 M （S∕N ＝3）.인차，저충기우 HRP 최화화붕산특이성식별적방법위설계독특적생물전감기제공료일개량호적평태.
Carbon nanotube - HRP - poly (aniline boronic acid) (PABA) composites were compared based on enzyme catalyzed polymerization ,and a novel biosensor was constructed by spreading the composites on the carbon glass electrode .UV -visible and Fourier transform infrared spectra of the resulting MWCNTs - HRP - PABA composite films suggested that the immobilized HRP retained its original structure .Electrochemical impedance spectroscopy and scanning electron microscopy images of the sensor confirmed the formation of PABA by enzyme catalytic polymize in the presense of ABA -MWCHTs and HRP .Cyclic voltammograms of PABA at MWCNTs - HRP - PABA modified glassy carbon electrode showed a pair of well - defined redox peaks of PABA in 0.1 M PBS solution ,which indicated a direct electron transfer between the enzyme active center and the electrode surface .Amperometric measurement were linearly related to H2 O2 concentration on wider linearity range for 1.21 mM to 20 M with a correlation coefficient of 0.9973 , and the detection limit 5 M (S∕N = 3) . The enzymatically induced deposition of PABA provided a general platform for the design of novel electrochemical biosensors .