CAJ | 학술논문

探讨慢病毒介导的靶向 VEGF 小干扰 RNA 联合应用化疗药物5-FU 诱导人乳腺癌细胞 MCF-7凋亡的机制。以携带 VEGF siRNA 的慢病毒载体感染 MCF-7细胞，应用 RT-PCR 和 Western blot 分别检测各组VEGF mRNA、VEGF 蛋白及凋亡相关蛋白的表达，流式细胞术检测细胞凋亡。结果表明，慢病毒 VEGF siRNA干扰组细胞 VEGF mRNA 和蛋白表达水平明显低于对照组，凋亡相关蛋白 P53及 P21表达上调，而 SIRT1、Bcl-2及 Survivin 表达下调。流式细胞术检测显示慢病毒干扰组及5-FU 组细胞凋亡率显著升高，联合治疗组的协同作用更为明显。上述结果表明：慢病毒介导的 RNA 干扰能明显抑制 MCF-7细胞 VEGF 的表达，通过下调SIRTI 蛋白的表达，导致 P53蛋白表达上调，并调控其下游 P21、bcl-2和 Survivin 的表达，从而诱导 MCF-7细胞的凋亡，并且提高了 MCF-7对5-FU 的敏感性。
탐토만병독개도적파향 VEGF 소간우 RNA 연합응용화료약물5-FU 유도인유선암세포 MCF-7조망적궤제。이휴대 VEGF siRNA 적만병독재체감염 MCF-7세포，응용 RT-PCR 화 Western blot 분별검측각조VEGF mRNA、VEGF 단백급조망상관단백적표체，류식세포술검측세포조망。결과표명，만병독 VEGF siRNA간우조세포 VEGF mRNA 화단백표체수평명현저우대조조，조망상관단백 P53급 P21표체상조，이 SIRT1、Bcl-2급 Survivin 표체하조。류식세포술검측현시만병독간우조급5-FU 조세포조망솔현저승고，연합치료조적협동작용경위명현。상술결과표명：만병독개도적 RNA 간우능명현억제 MCF-7세포 VEGF 적표체，통과하조SIRTI 단백적표체，도치 P53단백표체상조，병조공기하유 P21、bcl-2화 Survivin 적표체，종이유도 MCF-7세포적조망，병차제고료 MCF-7대5-FU 적민감성。
The mechanism of the apoptosis of human breast cancer cell line MCF-7 induced by lentivirus with media-tion of target-oriented VEGF siRNA combined with the anticancer drug 5-FU was investigated. Lentiviral vectors carry-ing VEGF siRNA was infected into human breast cancer MCF-7 cells,the level of VEGF mRNA,VEGF protein and apoptosis related proteins of each group were measured by RT-PCR and Western blot respectively,cell apoptosis was analyzed by flow cytometry. The results showed that in the lentivirus VEGF siRNA interference group the VEGF mR-NA and protein expression level was significantly lower than that in the control group,the expression of the apoptosis related proteins P53 and P21 increased,while SIRT1,Bcl-2 and Survivin decreased. Flow cytometry results showed that the cell apoptosis rate in the interference group and 5-FU group were increased significantly,the synergistic effect of the combined treatment group was more obvious. The above-mentioned results indicated that lentivirus mediated siRNA interfering could significantly suppressed VEGF expression in MCF-7 cell,induced the apoptosis of MCF-7 cells by down-regulating the expression of SIRTl,and induced up-regulating the expression of P53,which regulated the expression of its down stream P21,Bcl-2 and Survivin,and induced the apoptosis of MCF-7,moreover improved the sensitivity of MCF-7 to 5-FU.