CAJ | 학술논문

酿酒酵母是第一个完成全基因组测序的真核生物，具有广泛的科研应用价值。利用酿酒酵母的全基因组序列可以进行精确的基因定位及敲除，从而达到对其基因组进行精简的目的，为合成生物学最小基因组的研究工作打下基础。根据 Latour system 设计敲除所需引物，构建敲除盒，筛选重组体和缺失体，成功敲除酿酒酵母 a 型单倍体染色体 XIII 中339301-352281 nt 包含的8个基因，为酿酒酵母染色体精简奠定基础，同时证明了 Latour system 可以应用于酿酒酵母大片段敲除。
양주효모시제일개완성전기인조측서적진핵생물，구유엄범적과연응용개치。이용양주효모적전기인조서렬가이진행정학적기인정위급고제，종이체도대기기인조진행정간적목적，위합성생물학최소기인조적연구공작타하기출。근거 Latour system 설계고제소수인물，구건고제합，사선중조체화결실체，성공고제양주효모 a 형단배체염색체 XIII 중339301-352281 nt 포함적8개기인，위양주효모염색체정간전정기출，동시증명료 Latour system 가이응용우양주효모대편단고제。
Saccharomyces cerevisiae is the first eucaryote to complete its whole genome sequencing;it has extensive application value of scientific research. Adopting the whole genome sequencing of S. cerevisiae could precisely conduct its gene location and deletion,accordingly to reach the goal of its genome simplification,therefore,lay down a founda-tion for the study on synthesis of an organism with minimal genome. In this paper,according to Latour system,prim-ers needed were precisely designed and deleted to construct a deletion cassette,and screen recombinant and deletant. Eight genes containing in 339301-352281 nt in chromosome XIII of monoploid S. cerevisiae type a were successfully deleted,and thereby laid down a foundation for chromosome simplification in S. cerevisiae. At the same time it had proved that Latour system can be applied in large fragment deletion in S. cerevisiae.