怀化学院学报
懷化學院學報
부화학원학보
JOURNAL OF HUAIHUA TEACHERS COLLEGE
2015年
5期
1-3
,共3页
清肺抑火片%栀子苷%大黄素%大黄酚%高效液相色谱法
清肺抑火片%梔子苷%大黃素%大黃酚%高效液相色譜法
청폐억화편%치자감%대황소%대황분%고효액상색보법
Qingfei Yihuo table%geniposide%emodin%chrysophanol%HPLC
采用 C18柱(250×4.6 mm ,5μm),梯度洗脱,以不同比例的乙腈-0.1%磷酸溶液为流动相,流速为1.0 mL∕min ,同时测定了清肺抑火片中栀子苷(238 nm)、大黄素(254 nm)、大黄酚(254 nm)的含量.栀子苷、大黄素和大黄酚分别在0.06~0.6μg 、0.08~0.8μg 和0.08~0.8μg 范围内呈现良好的线性关系.平均回收率分别为98.33%、97.43%和97.73%; RSD 值分别为0.55%、0.50%和0.87%.所建立的方法可同时测定清肺抑火片中栀子苷、大黄素、大黄酚的含量,可用于清肺抑火片的质量控制.
採用 C18柱(250×4.6 mm ,5μm),梯度洗脫,以不同比例的乙腈-0.1%燐痠溶液為流動相,流速為1.0 mL∕min ,同時測定瞭清肺抑火片中梔子苷(238 nm)、大黃素(254 nm)、大黃酚(254 nm)的含量.梔子苷、大黃素和大黃酚分彆在0.06~0.6μg 、0.08~0.8μg 和0.08~0.8μg 範圍內呈現良好的線性關繫.平均迴收率分彆為98.33%、97.43%和97.73%; RSD 值分彆為0.55%、0.50%和0.87%.所建立的方法可同時測定清肺抑火片中梔子苷、大黃素、大黃酚的含量,可用于清肺抑火片的質量控製.
채용 C18주(250×4.6 mm ,5μm),제도세탈,이불동비례적을정-0.1%린산용액위류동상,류속위1.0 mL∕min ,동시측정료청폐억화편중치자감(238 nm)、대황소(254 nm)、대황분(254 nm)적함량.치자감、대황소화대황분분별재0.06~0.6μg 、0.08~0.8μg 화0.08~0.8μg 범위내정현량호적선성관계.평균회수솔분별위98.33%、97.43%화97.73%; RSD 치분별위0.55%、0.50%화0.87%.소건립적방법가동시측정청폐억화편중치자감、대황소、대황분적함량,가용우청폐억화편적질량공제.
A simple and sensitive high - performance liquid chromatography (HPLC) analytical method for the simultaneous determination of Geniposide , Emodin ,Chrysophanol in Qingfei Yihuo table was developed .The analysis was performed on C18 (250 × 4.6 mm ,5 μm) column with gradient elution .The mobile phase was composed of acetonitrile -0.1% H3 PO4 ;The flow rate was 1.0 mL∕min - 1 .The calibration curves were linear with each other in the range of 0.06 ~ 0.6 μg for gardenoside (238 nm) ,0.08 ~ 0.8 μg for Emodin (254 nm) ,and 0.08 ~ 0.8 μg for Chrysophanol (254 nm) ; Average recovery was 98.33% , 97.43% ,97.73% ;RSD was 0.55% ,0.50% ,0.87% .This method was accurate ,rapid ,reproducible and suitable for the quality control of this preparation .