癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
CARCINOGENSES,TERATOGENSIS AND MUTAGENESIS
2015年
3期
230-233
,共4页
秦杰升%洪良利%林旭妮%王挥戈%邱前程
秦傑升%洪良利%林旭妮%王揮戈%邱前程
진걸승%홍량리%림욱니%왕휘과%구전정
喉鳞状细胞癌%脑信号蛋白4C%微小染色体维持蛋白2%免疫组化%微淋巴管密度
喉鱗狀細胞癌%腦信號蛋白4C%微小染色體維持蛋白2%免疫組化%微淋巴管密度
후린상세포암%뇌신호단백4C%미소염색체유지단백2%면역조화%미림파관밀도
laryngeal squamous carcinoma%Sema4C%minichromosome maintenance 2%immunohistochemistry%lymphatic microvessel density
目的：探讨脑信号蛋白4C(Sema4C)在喉鳞状细胞癌组织中的表达及意义。方法：采用免疫组化EnVision 二步法检测82例喉鳞状细胞癌及癌旁正常喉黏膜组织中Sema4C和微小染色体维持蛋白2(MCM2)蛋白的表达水平，用D2-40标记淋巴管，计数微淋巴管密度(LMVD)，分析三者的表达水平变化与临床病理指标的关系。结果：Sema4C、MCM2蛋白在喉鳞状细胞癌组织中的阳性率分别为60%(49/82)及98%(80/82)，均显著高于癌旁正常喉黏膜组织( P<0.01)， LMVD在癌及癌旁喉黏膜中的差别无统计学意义( P=0.077)； Sema4C、MCM2的表达水平与颈部淋巴结转移有关，与年龄及肿瘤T分级无显著相关。Sema4C、MCM2蛋白表达水平和LMVD，两两间均呈正相关关系(P<均0.05)。结论：Sema4C的异常高表达与喉鳞状细胞癌的发生发展、转移及预后有关，提示Sema4C有可能成为判断喉鳞状细胞癌生物学行为及预后指标。
目的：探討腦信號蛋白4C(Sema4C)在喉鱗狀細胞癌組織中的錶達及意義。方法：採用免疫組化EnVision 二步法檢測82例喉鱗狀細胞癌及癌徬正常喉黏膜組織中Sema4C和微小染色體維持蛋白2(MCM2)蛋白的錶達水平，用D2-40標記淋巴管，計數微淋巴管密度(LMVD)，分析三者的錶達水平變化與臨床病理指標的關繫。結果：Sema4C、MCM2蛋白在喉鱗狀細胞癌組織中的暘性率分彆為60%(49/82)及98%(80/82)，均顯著高于癌徬正常喉黏膜組織( P<0.01)， LMVD在癌及癌徬喉黏膜中的差彆無統計學意義( P=0.077)； Sema4C、MCM2的錶達水平與頸部淋巴結轉移有關，與年齡及腫瘤T分級無顯著相關。Sema4C、MCM2蛋白錶達水平和LMVD，兩兩間均呈正相關關繫(P<均0.05)。結論：Sema4C的異常高錶達與喉鱗狀細胞癌的髮生髮展、轉移及預後有關，提示Sema4C有可能成為判斷喉鱗狀細胞癌生物學行為及預後指標。
목적：탐토뇌신호단백4C(Sema4C)재후린상세포암조직중적표체급의의。방법：채용면역조화EnVision 이보법검측82례후린상세포암급암방정상후점막조직중Sema4C화미소염색체유지단백2(MCM2)단백적표체수평，용D2-40표기림파관，계수미림파관밀도(LMVD)，분석삼자적표체수평변화여림상병리지표적관계。결과：Sema4C、MCM2단백재후린상세포암조직중적양성솔분별위60%(49/82)급98%(80/82)，균현저고우암방정상후점막조직( P<0.01)， LMVD재암급암방후점막중적차별무통계학의의( P=0.077)； Sema4C、MCM2적표체수평여경부림파결전이유관，여년령급종류T분급무현저상관。Sema4C、MCM2단백표체수평화LMVD，량량간균정정상관관계(P<균0.05)。결론：Sema4C적이상고표체여후린상세포암적발생발전、전이급예후유관，제시Sema4C유가능성위판단후린상세포암생물학행위급예후지표。
OBJECTIVE:To investigate the expression of semaphorin 4C(Sema4C) in laryngeal squamous carcinoma and its role in tumor proliferation and lymphatic metastasis. METHODS:We investigated the expression of Sema4C and MCM2 in 82 cases of laryngeal squamous carcinoma and normal laryngeal tissues by immunohistochemical technique.Furthermore,we used D2-40 to label the lymphatic microvessel and measure the lymphatic microvessel density(LMVD) to analyze their roles in laryngeal squamous carcinoma.RESULTS:The expressions of Sema4C and MCM2 in laryngeal squamous carcinoma were significantly higher than that in the normal laryngeal tissues (P<0.01),but the difference of LVMD was not significant (P=0.077). The aberrant expression rates of Sema4C,MCM2 and LVMD in laryngeal squamous carcinoma were closely related to lymph node metastasis but did not correlate with age and T stage. LMVD in laryngeal squamous carcinoma should positive correlation with the expressions of Sema4C (P<0.05) and MCM2 (P<0.01). Moreover,there was positive correlation between Sema4C and MCM2 (P<0.05).CONCLUSION:Sema4C may play a role in the development of laryngeal squamous carcinoma. It may be possible to follow the progression of the disease and provide prognosis by detecting the expression of Sema4c.
