癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
CARCINOGENSES,TERATOGENSIS AND MUTAGENESIS
2015年
3期
207-210,215
,共5页
欧晓燕%刘新霞%蒋骏%陆垚%赵志强%冯简青%孙易%杨亚蕊%崔栋%陈静莉%申碧羚%邢秀梅%何云
歐曉燕%劉新霞%蔣駿%陸垚%趙誌彊%馮簡青%孫易%楊亞蕊%崔棟%陳靜莉%申碧羚%邢秀梅%何雲
구효연%류신하%장준%륙요%조지강%풍간청%손역%양아예%최동%진정리%신벽령%형수매%하운
彗星试验%血液%职业人群%DNA损伤
彗星試驗%血液%職業人群%DNA損傷
혜성시험%혈액%직업인군%DNA손상
comet assay%blood%occupational groups%DNA damage
目的：研究电子行业不同工种职业人群的DNA损伤情况，为确定健康危害预防重点提供依据。方法：选择珠三角某电子厂在职工人563名作为职业接触组，其中铅暴露组( n=167)，异丙醇组( n=287)，噪声组( n=39)，铅+高温组( n=34)，异丙醇+高温组(n=36)；选取就业前体检工人作为对照组( n=90)。采集人体微量外周血进行彗星试验，运用倒置荧光显微镜拍摄彗星图片，并通过CASP彗星分析软件对彗星图片进行分析。结果：铅+高温组工人外周血的核DNA含量低于对照组(P<0.05)，尾DNA含量、尾长和尾矩高于对照组(P<0.05)；异丙醇+高温组工人外周血的核DNA含量低于对照组(P<0.05)，尾DNA含量、尾长、彗星全长、尾惯量和尾矩高于对照组(P<0.05)。结论：本研究所选取的电子行业各工种工人均有明显的DNA损伤情况，尤以异丙醇+高温组工种最为严重，其次是铅+高温组工种，应引起职业病防治管理机构和厂方的特别关注。
目的：研究電子行業不同工種職業人群的DNA損傷情況，為確定健康危害預防重點提供依據。方法：選擇珠三角某電子廠在職工人563名作為職業接觸組，其中鉛暴露組( n=167)，異丙醇組( n=287)，譟聲組( n=39)，鉛+高溫組( n=34)，異丙醇+高溫組(n=36)；選取就業前體檢工人作為對照組( n=90)。採集人體微量外週血進行彗星試驗，運用倒置熒光顯微鏡拍攝彗星圖片，併通過CASP彗星分析軟件對彗星圖片進行分析。結果：鉛+高溫組工人外週血的覈DNA含量低于對照組(P<0.05)，尾DNA含量、尾長和尾矩高于對照組(P<0.05)；異丙醇+高溫組工人外週血的覈DNA含量低于對照組(P<0.05)，尾DNA含量、尾長、彗星全長、尾慣量和尾矩高于對照組(P<0.05)。結論：本研究所選取的電子行業各工種工人均有明顯的DNA損傷情況，尤以異丙醇+高溫組工種最為嚴重，其次是鉛+高溫組工種，應引起職業病防治管理機構和廠方的特彆關註。
목적：연구전자행업불동공충직업인군적DNA손상정황，위학정건강위해예방중점제공의거。방법：선택주삼각모전자엄재직공인563명작위직업접촉조，기중연폭로조( n=167)，이병순조( n=287)，조성조( n=39)，연+고온조( n=34)，이병순+고온조(n=36)；선취취업전체검공인작위대조조( n=90)。채집인체미량외주혈진행혜성시험，운용도치형광현미경박섭혜성도편，병통과CASP혜성분석연건대혜성도편진행분석。결과：연+고온조공인외주혈적핵DNA함량저우대조조(P<0.05)，미DNA함량、미장화미구고우대조조(P<0.05)；이병순+고온조공인외주혈적핵DNA함량저우대조조(P<0.05)，미DNA함량、미장、혜성전장、미관량화미구고우대조조(P<0.05)。결론：본연구소선취적전자행업각공충공인균유명현적DNA손상정황，우이이병순+고온조공충최위엄중，기차시연+고온조공충，응인기직업병방치관리궤구화엄방적특별관주。
OBJECTIVE:To evaluate the DNA damage of different sub-groups of workers in the electronics industry,and to set up major preventive measures.METHODS:The 563 workers of an electronics factory in the Pearl River Delta Region were studied,according to their jobs we divided them into 5 groups,including lead exposure group (n=167),isopropyl alcohol group (n=287),noise group (n=39),lead+high temperature group (n=34),and isopro-panol+high temperature group (n=36),with 90 pre-employment workers as controls. DNA damage was detected using micro-whole blood specimen by the comet assay method.RESULTS:Nuclear DNA content of lead+high temperature group was lower than the control group (P<0.05),and its tail DNA content,tail length,and tail moment were higher than the control group (P<0.05). The nuclear DNA content of isopropyl alcohol+high temperature group was lower than the control group (P<0.05),the comet tail DNA content,tail length,comet length,olive tail moment and tail moment were higher than the control group (P<0.05).CONCLUSION:Workers of different types in electronics industry chosen in this study had obvious DNA damage,especially isopropanol+high temperature group,followed by lead+high temperature group,to whichspecial attention should be paid to these.
