CAJ | 학술논문

目的：比较两种荧光波长CdSeS/ZnS-COOH合金量子点对细胞微核组效应遗传毒作用的特征。方法：采用L5178Y细胞胞质分裂阻滞微核细胞组学试验，490和540 nm两种荧光波长的合金量子点CdSeS/ZnS-COOH受试浓度均为0.0625、0.125、0.25、0.5和0.1 mg/mL，观察其微核组效应、剂量-效应和时间-效应关系。结果：与阴性对照组相比，两种波长的CdSeS/ZnS-COOH合金量子点在0.0625 mg/mL剂量时均可诱导微核率增加(P<0.05)；两者都能诱导Ⅰ型微核、Ⅱ型微核和核芽效应，但490 nm合金量子点不能诱导核质桥效应，而540 nm合金量子点能诱导核质桥效应。490 nm合金量子点在0.0625 mg/mL可诱导产生总微核、Ⅰ型微核和核芽，而540 nm合金量子点在此浓度仅可诱导产生Ⅰ型微核；两者均随着剂量进一步增加诱导产生其他效应；各种微核组效应有明显的剂量-效应关系(P<0.05)。490 nm量子点在9 h首先出现核质桥，540 nm量子点在9 h首先出现总微核和Ⅱ型微核数增加；随时间延长进一步出现其他效应，27 h各效应值达到峰值，此后下降。结论：两种荧光波长CdSeS/ZnS-COOH合金量子点均可诱导微核组学效应，但在相同剂量的效应谱、剂量-效应和时间-效应方面均有差异，表明两者的遗传毒作用特点有差异。
목적：비교량충형광파장CdSeS/ZnS-COOH합금양자점대세포미핵조효응유전독작용적특정。방법：채용L5178Y세포포질분렬조체미핵세포조학시험，490화540 nm량충형광파장적합금양자점CdSeS/ZnS-COOH수시농도균위0.0625、0.125、0.25、0.5화0.1 mg/mL，관찰기미핵조효응、제량-효응화시간-효응관계。결과：여음성대조조상비，량충파장적CdSeS/ZnS-COOH합금양자점재0.0625 mg/mL제량시균가유도미핵솔증가(P<0.05)；량자도능유도Ⅰ형미핵、Ⅱ형미핵화핵아효응，단490 nm합금양자점불능유도핵질교효응，이540 nm합금양자점능유도핵질교효응。490 nm합금양자점재0.0625 mg/mL가유도산생총미핵、Ⅰ형미핵화핵아，이540 nm합금양자점재차농도부가유도산생Ⅰ형미핵；량자균수착제량진일보증가유도산생기타효응；각충미핵조효응유명현적제량-효응관계(P<0.05)。490 nm양자점재9 h수선출현핵질교，540 nm양자점재9 h수선출현총미핵화Ⅱ형미핵수증가；수시간연장진일보출현기타효응，27 h각효응치체도봉치，차후하강。결론：량충형광파장CdSeS/ZnS-COOH합금양자점균가유도미핵조학효응，단재상동제량적효응보、제량-효응화시간-효응방면균유차이，표명량자적유전독작용특점유차이。
OBJECTIVE:To compare the genotoxic characteristics of micronucleus cytomic effect of the two differential fluorescence wave lengths CdSeS/ZnS-COOH alloyed quantum dots(QDs).METHODS:Using the cytokinesis block micronucleus cytomic assays as the major method,mouse lymphoma cells (L5178Y) were put under the test doses of 0.062 5,0.125,0.25,0.5 and 0.1 mg/mL,the micronucleus cytomic effect,dose-effect and time-effect relationships were assessed. RESULTS:Compared with the negative control group,the two wavelengths of CdSeS/ZnS-COOH alloyed QDs at 0.062 5 mg/mL could induce the emergence of micronucleus(P<0.05). Both could induce types Ⅰ and Ⅱ micronucleus and nuclear buds effects,but 490 nm alloyed QDs could not induce nucleoplasmic bridge effect,while 540 nm alloyed QDs could. 490 nm alloyed QDs at the lowest dose could induce total micronucleus,typeⅠ micronucleus and nuclear buds,whilst 540 nm alloyed QDs at the lowest dose could induce typeⅠmicronucleus. With the increasing dose other effects emerged. The micronucleus cytomic effect of various groups had a clear dose-response relationship(P<0.05). 490 nm QDs could induce the emergence of nucleoplasmic bridges first after 9 h exposure,while 540 nm QDs induced the increase of total micronucleus and type Ⅱ micronucleus first. As time increased other effects began to develop,and reached their peaks at 27 h,then declined.CONCLUSION:Both QDs could induce micronuclus cytomic effect,but at the same dose their spectrum of effect,dose-effect and time-effect were different,which showed that they possessed different genotoxic characteristics.