CAJ | 학술논문

目的：探讨人肺癌细胞株H1299过表达亲环素A(CyPA)后对高温、紫外线和顺铂作用引起的细胞凋亡的影响。方法：采用重组慢病毒介导构建CyPA过表达H1299细胞株H1299 CyPA OE，并用Western blot方法验证CyPA过表达细胞株是否成功构建。培养H 1299和H 1299 C y P A O E细胞株，分别进行高温(44℃处理60 m i n )、紫外线(90μW/c m 2处理60 m i n )和顺铂(37.5、75和150μg/m L作用2 h)处理后，用高内涵分析平台检测细胞的晚期凋亡。结果：H1299 CyPA OE细胞能检测到绿色荧光，且其CyPA蛋白表达较H1299细胞明显增高，表明H1299 CyPA OE细胞株构建成功。在不同顺铂浓度下，H1299 CyPA OE细胞凋亡率较H1299细胞均明显降低(P均<0.05)，而在高温和紫外线作用下，H1299 CyPA OE细胞凋亡率与H1299细胞相比差异均无统计学意义(P均>0.05)。结论：CyPA过表达对顺铂诱导的凋亡有抵抗作用，表明CyPA可作为潜在的联合化疗的基因靶点，有望为临床个体化治疗提供新的思路。
목적：탐토인폐암세포주H1299과표체친배소A(CyPA)후대고온、자외선화순박작용인기적세포조망적영향。방법：채용중조만병독개도구건CyPA과표체H1299세포주H1299 CyPA OE，병용Western blot방법험증CyPA과표체세포주시부성공구건。배양H 1299화H 1299 C y P A O E세포주，분별진행고온(44℃처리60 m i n )、자외선(90μW/c m 2처리60 m i n )화순박(37.5、75화150μg/m L작용2 h)처리후，용고내함분석평태검측세포적만기조망。결과：H1299 CyPA OE세포능검측도록색형광，차기CyPA단백표체교H1299세포명현증고，표명H1299 CyPA OE세포주구건성공。재불동순박농도하，H1299 CyPA OE세포조망솔교H1299세포균명현강저(P균<0.05)，이재고온화자외선작용하，H1299 CyPA OE세포조망솔여H1299세포상비차이균무통계학의의(P균>0.05)。결론：CyPA과표체대순박유도적조망유저항작용，표명CyPA가작위잠재적연합화료적기인파점，유망위림상개체화치료제공신적사로。
OBJECTIVE:This study aimed to explore the effects of cyclophilin A (CyPA) over-expression in human lung adenocarcinoma cell line H1299 after treatments of high temperature,ultraviolet (UV) and cisplatin-induced apoptosis.METHODS:We set up H1299 CyPA over-expression cell lines via lentiviral transfection and used fluorescence microscope and Western blot analysis to analyze the expression of the target gene. H1299 and H1299 CyPA OE cell lines were cultured and exposed to treatments of high temperature,UV and cisplatin. High intension analysis platform was used to detect apoptosis of the two cell lines.RESULTS:Green fluorescent could be detected in H1299 CyPA OE cell line,and the protein CyPA expression was much higher than H1299 cell line,indicating that H1299 CyPA OE cell line was set up successfully. The apoptosis rate of H1299 CyPA OE cell line was much lower than H1299 cell line under different concentrations of cisplatin(P<0.05),but meanwhile the difference of apoptosis rates of H1299 CyPA OE cell line and H1299 cell lines showed no statistical significance(P>0.05).CONCLUSION:The over-expression of CyPA could confer resistance to apoptosis induced by cisplatin. CyPA may act as a potential gene target and provide new strategies of combined chemotherapy for lung cancer.