化学与生物工程
化學與生物工程
화학여생물공정
CHEMISTRY & BIOENGINEERING
2015年
5期
52-54,60
,共4页
米曲霉%曲酸%常压室温等离子体诱变%突变株%高通量筛选
米麯黴%麯痠%常壓室溫等離子體誘變%突變株%高通量篩選
미곡매%곡산%상압실온등리자체유변%돌변주%고통량사선
Aspergillus oryzae%kojic acid%atmospheric and room temperature plasma (ARTP)%mutant strain%high-throughput screening
以米曲霉 KA2308作为出发菌株，首次利用常压室温等离子体诱变技术选育高产曲酸菌株，通过96孔板高通量筛选获得一株曲酸生产菌 KA1515，曲酸产量提高到47.28 g·L－1，比出发菌株提高了31.48％，经过多次传代，曲酸产量仍比较稳定。表明采用常压室温等离子体诱变能有效提高米曲霉曲酸产量。
以米麯黴 KA2308作為齣髮菌株，首次利用常壓室溫等離子體誘變技術選育高產麯痠菌株，通過96孔闆高通量篩選穫得一株麯痠生產菌 KA1515，麯痠產量提高到47.28 g·L－1，比齣髮菌株提高瞭31.48％，經過多次傳代，麯痠產量仍比較穩定。錶明採用常壓室溫等離子體誘變能有效提高米麯黴麯痠產量。
이미곡매 KA2308작위출발균주，수차이용상압실온등리자체유변기술선육고산곡산균주，통과96공판고통량사선획득일주곡산생산균 KA1515，곡산산량제고도47.28 g·L－1，비출발균주제고료31.48％，경과다차전대，곡산산량잉비교은정。표명채용상압실온등리자체유변능유효제고미곡매곡산산량。
The strain of Aspergillus oryzae KA2308 was exposed on atmospheric and room temperature plasma(ARTP)jet to improve kojic acid production for the first time.A high kojic acid-producing strain KA1515 was obtained by high-throughput screening method with 96-well plates.The yield of kojic acid of mu-tant reached up to 47.28 g·L-1 ,increasing by 31.48% compared with original strain.Kojic acid yield was sta-ble after several passages.The results showed that mutation by atmospheric and room temperature plasma could raise kojic acid yield of Aspergillus oryzae effectively.
