CAJ | 학술논문

目的：研究肺结核患者支气管肺泡灌洗液( BALF)中γ干扰素( IFN-γ)、白细胞介素12( IL-12)、白细胞介素4(IL-4)、白细胞介素10(IL-10)水平的变化及其临床意义。方法采用双抗体夹心ELISA法检测60例活动性肺结核患者、33例非活动性肺结核患者BALF中IFN-γ、IL-12、IL-4、IL-10的水平,对其中32例活动性肺结核患者抗结核治疗后的上述细胞因子水平进行随访。组间比较采用t检验。结果活动性肺结核病例BALF中IFN-γ、IL-12的水平要显著高于非活动性肺结核病例(P<0．01),而IL-4、IL-10的水平两组间无明显差异( P >0．05)。菌阳及重症肺结核的 IFN-γ、IL-4、IL-12水平要明显高于菌阴及轻症( P <0．05),IL-10值无明显差异(P>0．05)。32例活动性肺结核的病例在抗痨治疗一个月后复查支气管镜,BALF中IFN-γ、IL-4、IL-12的水平均较治疗前有明显的下降( P<0．01)。而IL-10值亦有所下降,但差异无统计学意义(P>0．05)。结论肺结核患者BALF中IFN-γ、IL-4、IL-12水平的检测可作为了解病灶活动性、严重程度、监测疗效的参考。
목적：연구폐결핵환자지기관폐포관세액( BALF)중γ간우소( IFN-γ)、백세포개소12( IL-12)、백세포개소4(IL-4)、백세포개소10(IL-10)수평적변화급기림상의의。방법채용쌍항체협심ELISA법검측60례활동성폐결핵환자、33례비활동성폐결핵환자BALF중IFN-γ、IL-12、IL-4、IL-10적수평,대기중32례활동성폐결핵환자항결핵치료후적상술세포인자수평진행수방。조간비교채용t검험。결과활동성폐결핵병례BALF중IFN-γ、IL-12적수평요현저고우비활동성폐결핵병례(P<0．01),이IL-4、IL-10적수평량조간무명현차이( P >0．05)。균양급중증폐결핵적 IFN-γ、IL-4、IL-12수평요명현고우균음급경증( P <0．05),IL-10치무명현차이(P>0．05)。32례활동성폐결핵적병례재항로치료일개월후복사지기관경,BALF중IFN-γ、IL-4、IL-12적수평균교치료전유명현적하강( P<0．01)。이IL-10치역유소하강,단차이무통계학의의(P>0．05)。결론폐결핵환자BALF중IFN-γ、IL-4、IL-12수평적검측가작위료해병조활동성、엄중정도、감측료효적삼고。
Objective To investigate the characteristics and its clinical value of IFN-γ, IL-12, IL-4 and IL-10 in bronchoalveolar lavage fluid ( BAIF) in patients with pulmonary tuberculosis. Methods The concentrations of IFN-γ, IL-12, IL-4 and IL-10 were measured by ELISA method in BAIF of 60 patients with active tuberculosis and 33 patients with inactive tuberculosis. Meanwhile, the above-mentioned cytokine levels in BALF of 32 patients with active tuberculosis were followed up. Differences between the two groups were assessed by t test. Results The levels of IFN-γand IL-12 in BAIF were significantly higher in the active tuberculosis group than in the inactive tuberculosis group (P<0. 01), and the levels of IL-4 and IL-10 showed no significant difference between the two groups (P>0. 05 ) . The levels of IFN-γ, IL-4 and IL-12 in BALF of the smear-positive and severe pulmonary tuberculosis groups were all significantly higher than those of the smear-negative and mild tuberculosis groups (P<0. 01). 1 month after anti-tuberculosis treatments, the levels of IFN-γ, IL-4 and IL-12 in BALF decreased significantly in 32 cases of ac-tive tuberculosis (P<0. 01). Conclusion The detection of IFN-γ, IL-4 and IL-12 in BALF is helpful to under-stand the activity and the severity of disease and monitor the therapeutic effect in patients with pulmonary tuberculo-sis.