山东大学学报(理学版)
山東大學學報(理學版)
산동대학학보(이학판)
JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE)
2015年
5期
88-94
,共7页
嗜盐聚磷菌%温度%pH%碳源%盐度
嗜鹽聚燐菌%溫度%pH%碳源%鹽度
기염취린균%온도%pH%탄원%염도
halophilic phosphate accumulating bacteria%temperature%pH%carbon sources%salinity
从运行稳定的以生活污水为碳源的污泥中富集分离,并筛选出3株嗜盐聚磷菌菌株:qdp01、菌株 qdp03和菌株 qdp05。通过对菌株的形态、生理生化特征及16S rDNA 序列进行分析后,确定菌株 qdp01属于产碱杆菌属(Al-caligenes sp.);菌株 qdp03和菌株 qdp05为肠杆菌属(Enterobacter sp.)。通过改变温度、pH、碳源、盐度等环境因素,研究以上因素对3株菌除磷效果的影响,得出了3株菌除磷的最佳环境条件。当温度为30℃、pH 值在7.5~9.0、碳源为乙酸钠、盐度为2%时,3株菌对磷的去除率均可达到80%以上。特别研究了不同盐度对菌株生长和除磷性能的影响,结果显示3株菌在高盐环境中磷的去除有较高的应用价值。
從運行穩定的以生活汙水為碳源的汙泥中富集分離,併篩選齣3株嗜鹽聚燐菌菌株:qdp01、菌株 qdp03和菌株 qdp05。通過對菌株的形態、生理生化特徵及16S rDNA 序列進行分析後,確定菌株 qdp01屬于產堿桿菌屬(Al-caligenes sp.);菌株 qdp03和菌株 qdp05為腸桿菌屬(Enterobacter sp.)。通過改變溫度、pH、碳源、鹽度等環境因素,研究以上因素對3株菌除燐效果的影響,得齣瞭3株菌除燐的最佳環境條件。噹溫度為30℃、pH 值在7.5~9.0、碳源為乙痠鈉、鹽度為2%時,3株菌對燐的去除率均可達到80%以上。特彆研究瞭不同鹽度對菌株生長和除燐性能的影響,結果顯示3株菌在高鹽環境中燐的去除有較高的應用價值。
종운행은정적이생활오수위탄원적오니중부집분리,병사선출3주기염취린균균주:qdp01、균주 qdp03화균주 qdp05。통과대균주적형태、생리생화특정급16S rDNA 서렬진행분석후,학정균주 qdp01속우산감간균속(Al-caligenes sp.);균주 qdp03화균주 qdp05위장간균속(Enterobacter sp.)。통과개변온도、pH、탄원、염도등배경인소,연구이상인소대3주균제린효과적영향,득출료3주균제린적최가배경조건。당온도위30℃、pH 치재7.5~9.0、탄원위을산납、염도위2%시,3주균대린적거제솔균가체도80%이상。특별연구료불동염도대균주생장화제린성능적영향,결과현시3주균재고염배경중린적거제유교고적응용개치。
Three halophilic phosphate accumulating bacterial strains qdp01,qdp03,qdp05 were separated and screened from the biological phosphorus removal sludge which was supplied with domestic sewage as carbon source under steady operation.The strain qdp01 were identified as Alcaligenes sp.by analyzing the morphology,physiology,biochemical features and 16S rDNA sequence.The strain qdp03 and qdp05 was identified as Enterobacter sp.Through researching their phosphorus removal by changing the temperature,pH,carbon sources and salinity,we get best environmental con-ditions for phosphorus removal.The results were as follows:when sodium acetate was used as carbon sources,tempera-ture 30 ℃,pH 7.5-9,salinity 2%,the removal rates of the PO3 -4 by the three strains were all over 80%.We especially researched the effect of different salinity on strains growth and phosphorus removal,so that the three strains have great application value for phosphorus removal of hyperhaline wastewater.