临床与病理杂志
臨床與病理雜誌
림상여병리잡지
International Journal of Pathology and Clinical Medicine
2015年
5期
767-771
,共5页
真菌%荧光染色%病理活检%改良Calcofluor White
真菌%熒光染色%病理活檢%改良Calcofluor White
진균%형광염색%병리활검%개량Calcofluor White
fungi%fuorescence stain%biopsy%modifed Calcofuor White
目的：探索对Calcofluor White荧光染色法的改良，为活检诊断真菌感染提供一种快速、经济、对比更清晰的染色方法。方法：采用Calcofluor White M2R和苏木素、伊红染液，组合成3种染色步骤，对32例病例进行染色，筛选出适用于活检组织，并且在光镜与荧光镜下能同时观察的改良染色法步骤。结果：光镜下，组织呈现HE染色固有的形态。切换至荧光显微镜下，真菌则被显示为亮蓝色，组织背景呈黄绿色，结果清晰、醒目，对比强烈。结论：改良Calcofluor White荧光染色法能使同一张切片在光镜和荧光显微镜下同时观察，相互对照和定位，是提高真菌检出的好方法，值得在真菌的病理活检工作中广泛使用。
目的：探索對Calcofluor White熒光染色法的改良，為活檢診斷真菌感染提供一種快速、經濟、對比更清晰的染色方法。方法：採用Calcofluor White M2R和囌木素、伊紅染液，組閤成3種染色步驟，對32例病例進行染色，篩選齣適用于活檢組織，併且在光鏡與熒光鏡下能同時觀察的改良染色法步驟。結果：光鏡下，組織呈現HE染色固有的形態。切換至熒光顯微鏡下，真菌則被顯示為亮藍色，組織揹景呈黃綠色，結果清晰、醒目，對比彊烈。結論：改良Calcofluor White熒光染色法能使同一張切片在光鏡和熒光顯微鏡下同時觀察，相互對照和定位，是提高真菌檢齣的好方法，值得在真菌的病理活檢工作中廣汎使用。
목적：탐색대Calcofluor White형광염색법적개량，위활검진단진균감염제공일충쾌속、경제、대비경청석적염색방법。방법：채용Calcofluor White M2R화소목소、이홍염액，조합성3충염색보취，대32례병례진행염색，사선출괄용우활검조직，병차재광경여형광경하능동시관찰적개량염색법보취。결과：광경하，조직정현HE염색고유적형태。절환지형광현미경하，진균칙피현시위량람색，조직배경정황록색，결과청석、성목，대비강렬。결론：개량Calcofluor White형광염색법능사동일장절편재광경화형광현미경하동시관찰，상호대조화정위，시제고진균검출적호방법，치득재진균적병리활검공작중엄범사용。
Objective:To explore the improvement of Calcofluor White fluorescence staining and provide a fast and economical dyeing method with striking contrast for biopsy diagnosis of fungal infection. Methods:Calcolfuor White M2R, hematoxylin and eosin dyes were combined to form three different staining procedures and tostain 32 cases. The best procedure was selected, which was suitable for biopsy and for simultaneous observation of the staining under light microscope and lfuorescence microscope. Results:Tissue preserved morphology of HE staining under the light microscope. Switched to the lfuorescent microscope, the fungi were shown as bright blue, and the organizational background was displayed yellow to green. hTe results were clear, eye-catching and striking contrast. Conclusion:hTe modiifed Calcolfuor White lfuorescent staining method can make the same sections to be observed under light microscope and lfuorescence microscope simultaneously, easy to located and mutually control. It is a good method and worthy to be widely used for detecting fungi of biopsy.
