CAJ | 학술논문

目的：观察神经生长因子（NGF）对四氯化碳（CCL4）诱导的小鼠肝纤维化的影响。方法将30只雌性昆明小鼠分成3组：纤维化模型组（A组）、N G F干预组（B组）和生理盐水对照组（C组）。8周时采集标本，用全自动生化分析仪检测丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、总胆红素（TBIL）、清蛋白（ALB）；放射免疫法检测肝纤维化指标：透明质酸（H A ）、层粘连蛋白（L N ）、Ⅲ型前胶原（PCⅢ）。采用Ishaki评分系统对小鼠肝组织炎症活动度及纤维化程度进行评分。结果A、B组ALT、AST、HA、LN均明显高于C组（F＝111．45，658．80，157．43，167．99；P＜0．05）；B组ALT、AST、LN均明显低于A组（P＜0．05）。HE染色、网状纤维染色及Masson染色显示，A组肝组织炎症及纤维化程度最明显，B组肝组织炎症较A组显著减轻，未形成纤维间隔，纤维组织细短，C组肝组织未见明显炎症细胞浸润及纤维形成。A组肝脏炎症分级和纤维化分期评分较B、C组高，且3组炎症活动度得分、纤维化得分差异有统计学意义（均 P＜0．05）。结论 NGF可以阻断CCL4诱导的小鼠肝纤维化，减轻肝组织炎症。
목적：관찰신경생장인자（NGF）대사록화탄（CCL4）유도적소서간섬유화적영향。방법장30지자성곤명소서분성3조：섬유화모형조（A조）、N G F간예조（B조）화생리염수대조조（C조）。8주시채집표본，용전자동생화분석의검측병안산안기전이매（ALT）、천동안산안기전이매（AST）、총담홍소（TBIL）、청단백（ALB）；방사면역법검측간섬유화지표：투명질산（H A ）、층점련단백（L N ）、Ⅲ형전효원（PCⅢ）。채용Ishaki평분계통대소서간조직염증활동도급섬유화정도진행평분。결과A、B조ALT、AST、HA、LN균명현고우C조（F＝111．45，658．80，157．43，167．99；P＜0．05）；B조ALT、AST、LN균명현저우A조（P＜0．05）。HE염색、망상섬유염색급Masson염색현시，A조간조직염증급섬유화정도최명현，B조간조직염증교A조현저감경，미형성섬유간격，섬유조직세단，C조간조직미견명현염증세포침윤급섬유형성。A조간장염증분급화섬유화분기평분교B、C조고，차3조염증활동도득분、섬유화득분차이유통계학의의（균 P＜0．05）。결론 NGF가이조단CCL4유도적소서간섬유화，감경간조직염증。
Objective To observe the effect of nerve growth factor(NGF) on CCL4‐induced hepatic fibrosis in mice .Methods The hepatic fibrosis model was induced by subcutaneous injection of CCL 4 in mice .Thirty female Kunming mice were equally and randomly divided into three groups :fibrosis model group (A) ,NGF intervention group (B) and normal saline control group (C) .At 8 weeks following the initiation of experiment ,the samples were collected to measure ALT ,AST ,TBIL ,ALB by the fully automativ biochemical analyzer ,an the liver fibrosis indices (HA ,LN ,PC Ⅲ ) by radioimmunoassay .The Ishaki scoring system was adopted to assess the severity of hepatic inflammation and fibrosis degree .Results Serum levels of ALT ,AST ,HA and LN in the group A and B were significantly higher than those in the group C (F= 111 .45 ,658 .80 ,157 .43 ,167 .99 ;P< 0 .05) ,the levels of ALT 、AST and LN in the group B were significantly lower than those in the group A (P< 0 .05) .The HE staining ,reticular fiber staining and Masson staining showed that the liver fibrosis degree and the liver tissue inflammation in the group A were most obvious ,the liver tissue inflamation in the group B were significantly alleviated as compared with the group A .No fibrous septum was formed and the fiber tissues were fine and short .No obvious inflammatory cells infiltration and fibers formation were found in the liver tissue of the group C .The scores of liver inflamation grade and fibrosis staging in the group C were higher than those in the group B and C ,moreover the scores of liver inflammation grade and fibsosis had statstical differences among 3 groups (P < 0 .05) .Conclusion NGF can block hepatic fibrosis induced by CCL4 and relieve the liver inflammation .