重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2015年
15期
2020-2023
,共4页
吴得红%姚冬明%李云%林江%邓兆群%杨静%陈星星%钱震%马吉春%钱军
吳得紅%姚鼕明%李雲%林江%鄧兆群%楊靜%陳星星%錢震%馬吉春%錢軍
오득홍%요동명%리운%림강%산조군%양정%진성성%전진%마길춘%전군
慢性髓系白血病%let-7a-3%低甲基化%微小RNA
慢性髓繫白血病%let-7a-3%低甲基化%微小RNA
만성수계백혈병%let-7a-3%저갑기화%미소RNA
chronic myeloid leukemia%let-7a-3%hypomethylation%microRNA
目的：探讨慢性髓系白血病（CM L ）患者中let‐7a‐3启动子的甲基化态势及其临床意义。方法建立实时定量甲基化特异性PCR （RQ‐MSP）分别检测25例对照者及52例CML患者骨髓单个核细胞中let‐7a‐3启动子未甲基化水平。结果52例CM L患者未甲基化let‐7a‐3启动子（59．6％）为阳性，而对照组仅1例（4％）阳性，两组比较差异有统计学意义（ P＜0．01）。ROC曲线分析表明，let‐7a‐3启动子未甲基化作为辅助诊断CM L有较好的特异性。未甲基化let‐7a‐3启动子水平与BCR／ABL融合基因转录水平呈显著正相关（ r＝0．641，P＝0．001），但与患者的白细胞、血小板计数及血红蛋白水平无明显相关性（ P＞0．05）。在慢性期和加速期let‐7a‐3未甲基化水平显著高于急变期。结论 let‐7a‐3基因低甲基化水平随疾病进展而降低。
目的：探討慢性髓繫白血病（CM L ）患者中let‐7a‐3啟動子的甲基化態勢及其臨床意義。方法建立實時定量甲基化特異性PCR （RQ‐MSP）分彆檢測25例對照者及52例CML患者骨髓單箇覈細胞中let‐7a‐3啟動子未甲基化水平。結果52例CM L患者未甲基化let‐7a‐3啟動子（59．6％）為暘性，而對照組僅1例（4％）暘性，兩組比較差異有統計學意義（ P＜0．01）。ROC麯線分析錶明，let‐7a‐3啟動子未甲基化作為輔助診斷CM L有較好的特異性。未甲基化let‐7a‐3啟動子水平與BCR／ABL融閤基因轉錄水平呈顯著正相關（ r＝0．641，P＝0．001），但與患者的白細胞、血小闆計數及血紅蛋白水平無明顯相關性（ P＞0．05）。在慢性期和加速期let‐7a‐3未甲基化水平顯著高于急變期。結論 let‐7a‐3基因低甲基化水平隨疾病進展而降低。
목적：탐토만성수계백혈병（CM L ）환자중let‐7a‐3계동자적갑기화태세급기림상의의。방법건립실시정량갑기화특이성PCR （RQ‐MSP）분별검측25례대조자급52례CML환자골수단개핵세포중let‐7a‐3계동자미갑기화수평。결과52례CM L환자미갑기화let‐7a‐3계동자（59．6％）위양성，이대조조부1례（4％）양성，량조비교차이유통계학의의（ P＜0．01）。ROC곡선분석표명，let‐7a‐3계동자미갑기화작위보조진단CM L유교호적특이성。미갑기화let‐7a‐3계동자수평여BCR／ABL융합기인전록수평정현저정상관（ r＝0．641，P＝0．001），단여환자적백세포、혈소판계수급혈홍단백수평무명현상관성（ P＞0．05）。재만성기화가속기let‐7a‐3미갑기화수평현저고우급변기。결론 let‐7a‐3기인저갑기화수평수질병진전이강저。
Objective To investigate the methylation situation of let‐7a‐3 promoter in patients with chronic myeloid leukemia (CML) and its clinical significance .Methods The methylation level of let‐7a‐3 promoter in the bone marrow mononuclear cells of 52 CML patients and 25 controls was detected by using the real‐time quantitative methylation‐specific PCR (RQ‐PCR) .Results The non-hypomethylation of let‐7a‐3 promoter was positive in 31 cases(59 .6% ) of 52 CML patients ,while only 1 case(4% ,1/25) in the control group ,the difference between the two groups were statistically significant (P< 0 .01) .The ROC curve analysis showed that the non -hypomethylation of let‐7a‐3 has better specificity for the auxiliary diagnosis of CML .The significantly posi‐tive correlation was found between the non -methylation level of let‐7a‐3 promoter and the BCR/ABL transcription level (r=0 .641 ,P=0 .001) .In contrast ,there was no obvious correlation between the non -methylation level of let‐7a‐3 promoter and the WBC count ,platelet count and hemoglobin levels(P>0 .05) .The non-hypomethylation level of let‐7a‐3 in chronic phase and accel‐erate phase was significantly higher than that in blastic crisis of CML .Conclusion The hypomethylation level of let‐7a‐3 promoter is decreased with disease progression .
