中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2015年
6期
346-351
,共6页
刘敬一%邹晓英%陈雪%陈晔%岳林
劉敬一%鄒曉英%陳雪%陳曄%嶽林
류경일%추효영%진설%진엽%악림
牙乳头%干细胞%脂多糖类%基质细胞衍生因子1
牙乳頭%榦細胞%脂多糖類%基質細胞衍生因子1
아유두%간세포%지다당류%기질세포연생인자1
Dental papilla%Stem cells%Lipopolysaccharides%Stromal cell-derived factor-1
目的 明确人根尖牙乳头干细胞(stem cells from apical papilla,SCAP)中基质细胞衍生因子1(stromal cell-derived factor-1,SDF-1)的表达及脂多糖对其表达的影响.方法 分离培养18～24岁供者牙根未发育完全的人第三磨牙SCAP.实验分组:对照组加入细胞培养液,实验组加入含有大肠杆菌脂多糖的培养液,脂多糖终质量浓度分别为0.1、1.0及10.0 mg/L.采用细胞计数(cell counting kit-8,CCK-8)法检测脂多糖对SCAP增殖的影响,反转录PCR法检测SDF-1 mRNA在SCAP中的表达,实时PCR法检测24及48 h时脂多糖对SDF-1表达的影响.结果 脂多糖组细胞吸光度值(A值)与对照组在培养的前5天差异均无统计学意义,至第7天时脂多糖组细胞A值随脂多糖浓度增加显著降低;SCAP表达SDF-1 mRNA.在培养24 h时,0.1、1.0及10.0 mg/L脂多糖组SCAP中SDF-1mRNA的相对表达量分别为1.4±0.1、2.2±0.4及2.3±0.5,与未经脂多糖处理的对照组(设为1.0)相比差异均有统计学意义(F=12.102,P=0.002);48 h时0.1、1.0及10.0 mg/L脂多糖组SDF-1表达量(分别为2.1±0.4、3.4±0.3、3.8±0.5)随脂多糖浓度增加而升高的趋势愈加显著,与对照组相比差异均有统计学意义(F=39.054,P＜0.001).结论 SCAP具有趋化因子SDF-1的表达,脂多糖可在一定范围内刺激SDF-1的表达显著上调.
目的 明確人根尖牙乳頭榦細胞(stem cells from apical papilla,SCAP)中基質細胞衍生因子1(stromal cell-derived factor-1,SDF-1)的錶達及脂多糖對其錶達的影響.方法 分離培養18～24歲供者牙根未髮育完全的人第三磨牙SCAP.實驗分組:對照組加入細胞培養液,實驗組加入含有大腸桿菌脂多糖的培養液,脂多糖終質量濃度分彆為0.1、1.0及10.0 mg/L.採用細胞計數(cell counting kit-8,CCK-8)法檢測脂多糖對SCAP增殖的影響,反轉錄PCR法檢測SDF-1 mRNA在SCAP中的錶達,實時PCR法檢測24及48 h時脂多糖對SDF-1錶達的影響.結果 脂多糖組細胞吸光度值(A值)與對照組在培養的前5天差異均無統計學意義,至第7天時脂多糖組細胞A值隨脂多糖濃度增加顯著降低;SCAP錶達SDF-1 mRNA.在培養24 h時,0.1、1.0及10.0 mg/L脂多糖組SCAP中SDF-1mRNA的相對錶達量分彆為1.4±0.1、2.2±0.4及2.3±0.5,與未經脂多糖處理的對照組(設為1.0)相比差異均有統計學意義(F=12.102,P=0.002);48 h時0.1、1.0及10.0 mg/L脂多糖組SDF-1錶達量(分彆為2.1±0.4、3.4±0.3、3.8±0.5)隨脂多糖濃度增加而升高的趨勢愈加顯著,與對照組相比差異均有統計學意義(F=39.054,P＜0.001).結論 SCAP具有趨化因子SDF-1的錶達,脂多糖可在一定範圍內刺激SDF-1的錶達顯著上調.
목적 명학인근첨아유두간세포(stem cells from apical papilla,SCAP)중기질세포연생인자1(stromal cell-derived factor-1,SDF-1)적표체급지다당대기표체적영향.방법 분리배양18～24세공자아근미발육완전적인제삼마아SCAP.실험분조:대조조가입세포배양액,실험조가입함유대장간균지다당적배양액,지다당종질량농도분별위0.1、1.0급10.0 mg/L.채용세포계수(cell counting kit-8,CCK-8)법검측지다당대SCAP증식적영향,반전록PCR법검측SDF-1 mRNA재SCAP중적표체,실시PCR법검측24급48 h시지다당대SDF-1표체적영향.결과 지다당조세포흡광도치(A치)여대조조재배양적전5천차이균무통계학의의,지제7천시지다당조세포A치수지다당농도증가현저강저;SCAP표체SDF-1 mRNA.재배양24 h시,0.1、1.0급10.0 mg/L지다당조SCAP중SDF-1mRNA적상대표체량분별위1.4±0.1、2.2±0.4급2.3±0.5,여미경지다당처리적대조조(설위1.0)상비차이균유통계학의의(F=12.102,P=0.002);48 h시0.1、1.0급10.0 mg/L지다당조SDF-1표체량(분별위2.1±0.4、3.4±0.3、3.8±0.5)수지다당농도증가이승고적추세유가현저,여대조조상비차이균유통계학의의(F=39.054,P＜0.001).결론 SCAP구유추화인자SDF-1적표체,지다당가재일정범위내자격SDF-1적표체현저상조.
Objective To investigate the expression of stromal cell-derived factor-1(SDF-1) in human stem cells from apical papilla(SCAP),and to evaluate the effect of lipopolysaccharide(LPS) on SDF-1 expression by SCAP.Methods SCAP were isolated from dental papilla of human immature third molars.The expression of SDF-1 was evaluated by reverse transcription-PCR(RT-PCR).After SCAP being exposed to different concentrations(0.1,1.0,10 mg/L) of LPS for 24 and 48 h,the effect of LPS on cell proliferation and gene expression of SDF-1 was investigated by cell counting kit-8 and real-time PCR respectively,while cells without LPS stimulation were considered as negative control.Results LPS had no significant effect on SCAP proliferation until day 7.RT-PCR assays demonstrated that SCAP expressed SDF-1 mRNA.Different concentrations of LPS significantly promoted the SDF-1 expression in SCAP after 24 h(F=12.102,P=0.002) and 48 h(F=39.054,P＜0.001) exposure,with relative gene expression ratio(experimental/control) increased to 1.4±0.1,2.2±0.4,2.3±0.5 in 24 h group and 2.1±0.4,3.4±0.3,3.8±0.5 in 48 h group.Conclusions Isolated SCAP in cultures have the expression of SDF-1 mRNA.LPS can significantly promote the expression of SDF-1 in SCAP.
