国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2015年
2期
80-85
,共6页
宋闻%林先耀%王娟%吴亦栋%蒋茂莹%赵仕勇
宋聞%林先耀%王娟%吳亦棟%蔣茂瑩%趙仕勇
송문%림선요%왕연%오역동%장무형%조사용
肠道病毒感染%VP1基因%序列分析%点突变
腸道病毒感染%VP1基因%序列分析%點突變
장도병독감염%VP1기인%서렬분석%점돌변
Enterovirus infection%VP1 gene%Sequence analysis%Point mutation
目的 了解2013年杭州地区重症手足口病患儿感染的肠道病毒71型(EV71)分离株VP1区的基因特征.方法 采集2013年杭州市儿童医院收治的重症手足口病确诊病例粪便样本,用EV71特异性引物进行RT-PCR鉴定,选取EV71检测阳性的15例样本进行病毒分离并进行VP1基因的特异性扩增并测序,结合EV71各基因型及亚型的代表株构建系统进化树,并对VP1蛋白的氨基酸序列进行分析.结果 2013年该院分离的15株EV71分离株与A、B基因型参考株的核苷酸同源性分别为77.5％～79.8％、77.7％～83.1％,氨基酸同源性分别为92.8％～96.6％、92.8％～97.9％,与C基因型的核苷酸同源性为83.4％～97.3％,氨基酸同源性为93.4％～99.7％,其中与2008年安徽省阜阳市的EV71流行株[C4a (Fuyang-0508)]的核苷酸和氨基酸同源性最高,分别为95.0％～97.3％及96.2％～99.7％.系统进化树显示所有分离株与C4a亚型的代表株处于同一分支.15株EV71分离株与不同基因型代表株进行比对发现,在297个氨基酸位点中主要突变位点有11个.结论 2013年杭州地区重症手足口病患儿中流行的EV71均为C4a基因型,其VP1基因保守性好.
目的 瞭解2013年杭州地區重癥手足口病患兒感染的腸道病毒71型(EV71)分離株VP1區的基因特徵.方法 採集2013年杭州市兒童醫院收治的重癥手足口病確診病例糞便樣本,用EV71特異性引物進行RT-PCR鑒定,選取EV71檢測暘性的15例樣本進行病毒分離併進行VP1基因的特異性擴增併測序,結閤EV71各基因型及亞型的代錶株構建繫統進化樹,併對VP1蛋白的氨基痠序列進行分析.結果 2013年該院分離的15株EV71分離株與A、B基因型參攷株的覈苷痠同源性分彆為77.5％～79.8％、77.7％～83.1％,氨基痠同源性分彆為92.8％～96.6％、92.8％～97.9％,與C基因型的覈苷痠同源性為83.4％～97.3％,氨基痠同源性為93.4％～99.7％,其中與2008年安徽省阜暘市的EV71流行株[C4a (Fuyang-0508)]的覈苷痠和氨基痠同源性最高,分彆為95.0％～97.3％及96.2％～99.7％.繫統進化樹顯示所有分離株與C4a亞型的代錶株處于同一分支.15株EV71分離株與不同基因型代錶株進行比對髮現,在297箇氨基痠位點中主要突變位點有11箇.結論 2013年杭州地區重癥手足口病患兒中流行的EV71均為C4a基因型,其VP1基因保守性好.
목적 료해2013년항주지구중증수족구병환인감염적장도병독71형(EV71)분리주VP1구적기인특정.방법 채집2013년항주시인동의원수치적중증수족구병학진병례분편양본,용EV71특이성인물진행RT-PCR감정,선취EV71검측양성적15례양본진행병독분리병진행VP1기인적특이성확증병측서,결합EV71각기인형급아형적대표주구건계통진화수,병대VP1단백적안기산서렬진행분석.결과 2013년해원분리적15주EV71분리주여A、B기인형삼고주적핵감산동원성분별위77.5％～79.8％、77.7％～83.1％,안기산동원성분별위92.8％～96.6％、92.8％～97.9％,여C기인형적핵감산동원성위83.4％～97.3％,안기산동원성위93.4％～99.7％,기중여2008년안휘성부양시적EV71류행주[C4a (Fuyang-0508)]적핵감산화안기산동원성최고,분별위95.0％～97.3％급96.2％～99.7％.계통진화수현시소유분리주여C4a아형적대표주처우동일분지.15주EV71분리주여불동기인형대표주진행비대발현,재297개안기산위점중주요돌변위점유11개.결론 2013년항주지구중증수족구병환인중류행적EV71균위C4a기인형,기VP1기인보수성호.
Objective To investigate the genetic characteristics of the VP1 gene,enterovirus 71 (EV71) of isolated strains in Hangzhou in 2013.Methods To collect the stool specimens of the confirmed cases of severe HFMD from Hangzhou Children's Hospital in 2013.All of the specimens were identified by reverse transcription polymerase chain reaction (RT-PCR) with EV71-type-specific primers.Full length VP1 genes of EV71 isolated strains from 15 positive specimens were specifically amplified by RT-PCR and sequenced.The sequences were compared with VP1 genes from EV71 in GenBank database.The amino acid sequence of VP1 protein was analyzed.Results The nucleotide homogeneities between these 15 Hangzhou strains and the representative isolates of genotype A and B were 77.5％-79.8％ and 77.7％-83.1％,respectively;while the amino acid homogeneities were 92.8％-96.6％ and 92.8％-97.9％,respectively.The nucleotide and amino acid homogeneities between the 15 Hangzhou strains and the representative isolates of genotype C were 83.4％-97.3％ and 93.4％-99.7％,respectively.The 15 Hangzhou strains had the closest homology with C4a (Fuyang-0508) strain in 2008,of which the nucleotide and amino acid homogeneities appeared to be 95％-97.3％ and 96.2％-99.7％,respectively.The 15 EV71 Hangzhou strains were at the same branch as the representative strain of subgenogroup C4a in the phylogenetic tree.There were 11 main point mutations from the total 279 amino acid points between the 15 Hangzhou strains and other representative strains.Conclusions The EV71 strains isolated from severe HFMD of Hangzhou in 2013 belong to subgenogroup C4a,and the VP1 gene is conservative.
