CAJ | 학술논문

目的探讨白癜风患者血清中干扰素(IFN-γ)-γ2、白细胞介素(IL)-4、转化生子因子α(TGF-α)、肿瘤坏死因子-β(TNF-β)表达水平和病变组织中切除修复交叉互补蛋白(ERCC1)、γ-H2AX蛋白表达变化与白癜风分期的关系.方法酶联免疫吸附试验(ELISA)检测不同分期白癜风患者血清中IFN-γ、IL-4、TGF-α、TNF-β表达水平的变化,蛋白印记(Western blot)检测病变组织中ERCC1和γ-H2AX蛋白表达变化.结果稳定期和进展期白癜风患者与对照组相比,患者血清中IFN-γ和TGF-β表达水平降低(F=37.48,F=42.41,P＜0.05),IL-4和TNF-β表达水平显著增加(F=56.37,F=77.19,P＜0.05).进展期白癜风患者与对照组相比,患者血清的IFN-γ/IL-4比值降低(F=46.27,P＜0.05);稳定期和进展期白癜风患者与对照组相比,病变组织中修复交叉互补蛋白(ERCC1)蛋白水平降低(F =45.71,P＜0.05),而γ-H2AX蛋白水平显著增高(F =37.96,P＜0.05).结论 IFN-y、IL-4、TGF-α和TNF-β参与白癜风的发病过程,ERCC1和γ-H2AX蛋白与白癜风病理损伤密切相关.
목적 탐토백전풍환자혈청중간우소(IFN-γ)-γ2、백세포개소(IL)-4、전화생자인자α(TGF-α)、종류배사인자-β(TNF-β)표체수평화병변조직중절제수복교차호보단백(ERCC1)、γ-H2AX단백표체변화여백전풍분기적관계.방법 매련면역흡부시험(ELISA)검측불동분기백전풍환자혈청중IFN-γ、IL-4、TGF-α、TNF-β표체수평적변화,단백인기(Western blot)검측병변조직중ERCC1화γ-H2AX단백표체변화.결과 은정기화진전기백전풍환자여대조조상비,환자혈청중IFN-γ화TGF-β표체수평강저(F=37.48,F=42.41,P＜0.05),IL-4화TNF-β표체수평현저증가(F=56.37,F=77.19,P＜0.05).진전기백전풍환자여대조조상비,환자혈청적IFN-γ/IL-4비치강저(F=46.27,P＜0.05);은정기화진전기백전풍환자여대조조상비,병변조직중수복교차호보단백(ERCC1)단백수평강저(F =45.71,P＜0.05),이γ-H2AX단백수평현저증고(F =37.96,P＜0.05).결론 IFN-y、IL-4、TGF-α화TNF-β삼여백전풍적발병과정,ERCC1화γ-H2AX단백여백전풍병리손상밀절상관.
Objective To explore the relationship between the expression of interferon-γ(IFN-γ),interleukin (IL)-4,transforming growth factor-α (TGF-α) and tumor necrosis factor-β(TNF-β) in serum and excision repair cross complementing-1 (ERCC1) and γ-H2AX protein in pathological tissues of vitiligo patients.Methods Enzyme-linked immunosorbent assay (ELISA)was used to detect the expression of IFN-γ,IL-4,TGF-α and TNF-β in serum of vitiligo patients with different phases.Western blot was used to detect the ERCC1 and γ-H2AX protein in pathological tissues of vitiligo patients.Results Compared with control,the expression of IFN-γ and TGF-β was decreased(F =37.48,F =42.41,P ＜ 0.05),while the expression of IL-4 and TNF-β was increased in serum of vitiligo patients in stable and progressive phase(F =56.37,F =77.19,P ＜ 0.05).Compared with control,the ratio of IFN-γ/IL-4 decreased in vitiligo patients in progressive phase (F =46.27,P ＜ 0.05).Compared with control,the expression of excision repair cross complementing 1 ERCC1 protein was significantly decreased(F =45.71,P ＜ 0.05),while γ-H2AX protein was significantly increased in pathological tissues of vitiligo patients in stable and progressive phase (F =37.96,P ＜ 0.05).Conclusion IFN-γ,IL-4,TGF-α and TNF-β might take part in the pathogenesis of vitiligo.ERCC1 and γ-H2AX protein were associated with the pathological damage of vitiligo.