CAJ | 학술논문

目的了解柯萨奇病毒A16型(Cox A16)YY157株在多种细胞系中培养的增殖特性,为疫苗研制提供参考.方法把YY157株接种于非洲猴肾细胞(Vero)、人胚肺二倍体成纤维细胞MRC-5或KMB17进行适应性传代培养,观察细胞病变情况,检测病毒感染性滴度,绘制增殖曲线.结果 Cox A16 YY157株在3种细胞中培养均产生细胞病变,在Vero细胞中病变早,增殖快,滴度达8.5 lgCCID50/mL;在MRC-5细胞中,此毒株增殖速度比在Vero细胞中慢,滴度达7.5 lgCCID50/mL;在KMB17细胞中,此毒株增殖最慢,滴度只有7.0 1gCCID50/mL.结论 Cox A16型YY157株均可感染Vero、MRC-5和KMB17细胞并增殖,但敏感性和增殖速度有差异.
목적 료해가살기병독A16형(Cox A16)YY157주재다충세포계중배양적증식특성,위역묘연제제공삼고.방법 파YY157주접충우비주후신세포(Vero)、인배폐이배체성섬유세포MRC-5혹KMB17진행괄응성전대배양,관찰세포병변정황,검측병독감염성적도,회제증식곡선.결과 Cox A16 YY157주재3충세포중배양균산생세포병변,재Vero세포중병변조,증식쾌,적도체8.5 lgCCID50/mL;재MRC-5세포중,차독주증식속도비재Vero세포중만,적도체7.5 lgCCID50/mL;재KMB17세포중,차독주증식최만,적도지유7.0 1gCCID50/mL.결론 Cox A16형YY157주균가감염Vero、MRC-5화KMB17세포병증식,단민감성화증식속도유차이.
Objective To investigate the growth and proliferation features of coxsackievirus A16 (Cox A16) strain YY157 cultured in different cell lines,and provide a reference for the research of vaccine.Methods Cox A16 strain YY157 was inoculated into Vero,MRC-5 or KMB17 cells and adapted by passage culturing.Meanwhile,cytopathic effect (CPE) was observed,with infective titer of the virus determined and its multiplication curves drawn.Results Cox A16 strain YY157 could induce CPE in all three cell lines.In Vero cells,CPE appeared early and the virus propagated rapidly with its titer reached 8.50 lgCCID50/mL.In MRC-5 cells,the virus proliferated slower than that in Vero cells with its titer reached 7.5 lgCCID50/mL.In KMB17 cells,the growth rate of the virus was relatively lower with its titer only 7.0 LgCCID550/mL.Conclusions Cox A16 strain YY157 is able to infect and propagate in Vero,MRC-5 and KMB17 cell lines.However,the sensitivity of those cell lines to the strain and the proliferation rates of the virus in those cell lines are different.