CAJ | 학술논문

目的：通过骨灵膏及其拆方制剂对骨关节炎模型大鼠 IL -17、IL -18作用的研究，探讨骨灵膏及其拆方制剂对骨关节炎的作用机制。方法60只雌性 SD 大鼠随机分为骨灵膏组（GLG）、骨膏组（GUG）、灵膏组（LG）、塞来昔布组（CLXB）、模型组（M）及正常组（NG）共6组，冷固法建立模型，造模成功后，骨灵膏、骨膏、灵膏均按10.41 ml/（Kg·d）灌胃，塞来昔布以20.83 mg/（Kg· d）灌胃，其余组给予生理盐水灌胃。给药10周后，取大鼠血清及关节软骨，分别以免疫荧光和 ELISA法检测软骨 IL -17、IL -18阳性表达和血清 IL -17、IL -18的含量。结果 M 组与 NG 组比较能显著增加软骨细胞 IL -17、IL -18阳性表达及血清 IL -17、IL -18的含量（P ﹤0.01），GLG 与 M、GUG、LG 及 CLXB 组比较，能明显降低软骨细胞及血清 IL -17、IL -18阳性表达及含量（P ﹤0.01）。结论GLG 可能通过降低 OA 模型大鼠血清 IL -17、IL -18的含量及软骨细胞 IL -17、IL -18阳性表达，对抗 OA 的炎症损伤，保护了关节软骨，其作用明显优于其拆方制剂 GUG、LG 及西药 CLXB。
목적：통과골령고급기탁방제제대골관절염모형대서 IL -17、IL -18작용적연구，탐토골령고급기탁방제제대골관절염적작용궤제。방법60지자성 SD 대서수궤분위골령고조（GLG）、골고조（GUG）、령고조（LG）、새래석포조（CLXB）、모형조（M）급정상조（NG）공6조，랭고법건립모형，조모성공후，골령고、골고、령고균안10.41 ml/（Kg·d）관위，새래석포이20.83 mg/（Kg· d）관위，기여조급여생리염수관위。급약10주후，취대서혈청급관절연골，분별이면역형광화 ELISA법검측연골 IL -17、IL -18양성표체화혈청 IL -17、IL -18적함량。결과 M 조여 NG 조비교능현저증가연골세포 IL -17、IL -18양성표체급혈청 IL -17、IL -18적함량（P ﹤0.01），GLG 여 M、GUG、LG 급 CLXB 조비교，능명현강저연골세포급혈청 IL -17、IL -18양성표체급함량（P ﹤0.01）。결론GLG 가능통과강저 OA 모형대서혈청 IL -17、IL -18적함량급연골세포 IL -17、IL -18양성표체，대항 OA 적염증손상，보호료관절연골，기작용명현우우기탁방제제 GUG、LG 급서약 CLXB。
Objective To study the effects of Gulinggao(GLG)paste and its split reagent prepara-tions(SRP)on IL - 18 and IL - 17 of rat model with osteoarthritis and explore the mechanism of action on Gulinggao Paste and its SRP to osteoarthritis. Methods A total of 60 female SD rats were randomly divided into normal group(NG),GLG group,Gugao group(GUG),Linggao group(LG),celecoxib(CLXB)group,and model group(M). Every groups used cold - solid method to establish model,and after the success of model-ing,LG,GUG,GLG all were administered intragastrically to rats with the amount of 10. 41 ml/(Kg·d). CLXB were given celecoxib 20. 83 mg/(Kg·)gavage,others were given physiological saline gavage. After 10 weeKs of administration,rat Knee serum and joint cartilage were taKen to detect the content and the positive expression of IL - 18 and IL - 17 in serum and cartilage with ELISA method and immunofluorescence respec-tively. Results M group compared with NG group could increase the content and positive expression of IL -18 and IL - 17 in serum and cartilage significantly(P ﹤ 0. 01);GLG compared with CLXB and M,GUG,LG group could reduce the content and positive expression of IL - 18 and IL - 17 in serum and cartilage obvious-ly(P ﹤ 0. 01). Conclusion GLG may fight the inflammation damage of OA through reducing the positive ex-pression and content of IL - 18 and IL - 17 in cartilage cells and serum on OA model rats,so as to protect the articular cartilage,and its effect is better than western medicine CLXB,GUG and LG.