中华眼视光学与视觉科学杂志
中華眼視光學與視覺科學雜誌
중화안시광학여시각과학잡지
CHINESE JOURNAL OF OPTOMETRY OPHTHALMOLOGY AND VISUAL SCIENCE
2015年
5期
284-287
,共4页
青光眼,开角型%小梁网%葡萄糖
青光眼,開角型%小樑網%葡萄糖
청광안,개각형%소량망%포도당
Glaucoma,open-angle%Trabecular meshwork%Glucose
目的 探讨不同浓度葡萄糖对POAG患者小梁网细胞凋亡的影响,研究葡萄糖与POAG以及糖尿病与POAG之间的关系,进一步探讨POAG的发病机制.方法 实验研究.采用组织块培养法原代培养POAG患者小梁网细胞,取传代的小梁网细胞分别加入含葡萄糖终浓度为0.0(对照组)、5.5、45.0 mmol/L的无血清培养基,分别培养48 h后,采用CCK-8和流式细胞仪法检测不同浓度葡萄糖对小梁网细胞的影响.数据采用单因素方差进行分析.结果 通过CCK-8检测发现,随着浓度的增加,葡萄糖对小梁网细胞的增殖抑制作用增强,可促进细胞凋亡,且各实验组与对照组之间相比差异有统计学意义(F=13.87,P＜0.01);通过流式细胞仪法检测葡萄糖浓度为5.5、45.0 mmol/L实验组小梁网细胞凋亡率分别为10.65％±0.03％、25.74％±0.02％,均高于对照组(4.02％±0.03％),且差异有统计学意义(F=25.34,P＜0.01).结论 高糖可以使小梁网细胞的增殖能力下降,凋亡率增加,从而可能导致小梁网细胞网状结构改变,房水流出途径的阻力增加.
目的 探討不同濃度葡萄糖對POAG患者小樑網細胞凋亡的影響,研究葡萄糖與POAG以及糖尿病與POAG之間的關繫,進一步探討POAG的髮病機製.方法 實驗研究.採用組織塊培養法原代培養POAG患者小樑網細胞,取傳代的小樑網細胞分彆加入含葡萄糖終濃度為0.0(對照組)、5.5、45.0 mmol/L的無血清培養基,分彆培養48 h後,採用CCK-8和流式細胞儀法檢測不同濃度葡萄糖對小樑網細胞的影響.數據採用單因素方差進行分析.結果 通過CCK-8檢測髮現,隨著濃度的增加,葡萄糖對小樑網細胞的增殖抑製作用增彊,可促進細胞凋亡,且各實驗組與對照組之間相比差異有統計學意義(F=13.87,P＜0.01);通過流式細胞儀法檢測葡萄糖濃度為5.5、45.0 mmol/L實驗組小樑網細胞凋亡率分彆為10.65％±0.03％、25.74％±0.02％,均高于對照組(4.02％±0.03％),且差異有統計學意義(F=25.34,P＜0.01).結論 高糖可以使小樑網細胞的增殖能力下降,凋亡率增加,從而可能導緻小樑網細胞網狀結構改變,房水流齣途徑的阻力增加.
목적 탐토불동농도포도당대POAG환자소량망세포조망적영향,연구포도당여POAG이급당뇨병여POAG지간적관계,진일보탐토POAG적발병궤제.방법 실험연구.채용조직괴배양법원대배양POAG환자소량망세포,취전대적소량망세포분별가입함포도당종농도위0.0(대조조)、5.5、45.0 mmol/L적무혈청배양기,분별배양48 h후,채용CCK-8화류식세포의법검측불동농도포도당대소량망세포적영향.수거채용단인소방차진행분석.결과 통과CCK-8검측발현,수착농도적증가,포도당대소량망세포적증식억제작용증강,가촉진세포조망,차각실험조여대조조지간상비차이유통계학의의(F=13.87,P＜0.01);통과류식세포의법검측포도당농도위5.5、45.0 mmol/L실험조소량망세포조망솔분별위10.65％±0.03％、25.74％±0.02％,균고우대조조(4.02％±0.03％),차차이유통계학의의(F=25.34,P＜0.01).결론 고당가이사소량망세포적증식능력하강,조망솔증가,종이가능도치소량망세포망상결구개변,방수류출도경적조력증가.
Objective To investigate the effect of different concentrations of glucose on trabecular meshwork cells in patients with primary open-angle glaucoma (POAG);to study glucose and POAG as well as the relationship between diabetes and POAG;and to further understand the pathogenesis of POAG.Methods In this experimental study,human trabecular meshwork cells were first cultured and then subcultured.The cells were incubated with different doses of glucose (0,5.5,45.0 mmol/l)and the final concentration was diluted with DMEM/F12 without serum for 48 hours.CCK-8 and flow cytometry were used to study the effect of glucose on the apoptosis of the cultured trabecular meshwork cells.Data were analyzed using ANOVA.Results With an increase in glucose concentration,CCK-8detected the inhibition of trabecular meshwork cells.The difference between the experimental group and control group was statistically significant (F=13.87,P＜0.01).Flow cytometry showed that the apoptosis rates in the experimental group with the different doses of glucose (5.5,45.0 mmol/L) in the final concentrations were 10.65％±0.03％ and 25.74％±0.02％,which were higher than the control group (4.02％±0.03％).The difference between the experimental group and control group was statistically significant (F=25.34,P＜0.05).Conclusion High glucose levels can cause a decrease in the proliferation of trabecular meshwork cells and increase the rate of apoptosis.This changes the network structure of trabecular meshwork cells,and maybe changes the resistance in the aqueous outflow pathway.
