CAJ | 학술논문

目的：观察不同浓度雷公藤红素在不同时间对 AGS 细胞形态及细胞迁移的影响，探索雷公藤红素对 AGS 细胞迁移较佳抑制作用浓度及时间。方法将 AGS 细胞铺于6孔板并培养，待贴壁细胞密度达到70%～80%后，采用划痕法制造细胞迁移模型，分为 DMSO 及雷公藤红素5μM、1μM、0.5μM、0.1μM、0.01μM 浓度组，并分别于0 h、6 h、12 h、24 h 观察细胞的形态及细胞的迁移情况，且拍照记录，计算细胞迁移速度及细胞迁移抑制率并进行各组之间的比较。结果高浓度雷公藤红素使 AGS 细胞形态发生改变；雷公藤红素能够抑制 AGS 细胞迁移，且终浓度为1μM 时抑制作用最明显。同一浓度不同作用时间其对 AGS 细胞迁移的抑制作用差异有统计学意义（P ﹤0.05）；同一时间不同浓度雷公藤红素对 AGS 细胞迁移的抑制作用差异有统计学意义（P ﹤0.05）。结论高浓度雷公藤红素能够使 AGS 细胞形态发生改变并凋亡；雷公藤红素抑制了 AGS 细胞的迁移，且该抑制作用与浓度、时间相关，且在终浓度为1μM 作用于 AGS 细胞12 h 时，其对 AGS 细胞迁移的抑制作用最明显。
목적：관찰불동농도뢰공등홍소재불동시간대 AGS 세포형태급세포천이적영향，탐색뢰공등홍소대 AGS 세포천이교가억제작용농도급시간。방법장 AGS 세포포우6공판병배양，대첩벽세포밀도체도70%～80%후，채용화흔법제조세포천이모형，분위 DMSO 급뢰공등홍소5μM、1μM、0.5μM、0.1μM、0.01μM 농도조，병분별우0 h、6 h、12 h、24 h 관찰세포적형태급세포적천이정황，차박조기록，계산세포천이속도급세포천이억제솔병진행각조지간적비교。결과고농도뢰공등홍소사 AGS 세포형태발생개변；뢰공등홍소능구억제 AGS 세포천이，차종농도위1μM 시억제작용최명현。동일농도불동작용시간기대 AGS 세포천이적억제작용차이유통계학의의（P ﹤0.05）；동일시간불동농도뢰공등홍소대 AGS 세포천이적억제작용차이유통계학의의（P ﹤0.05）。결론고농도뢰공등홍소능구사 AGS 세포형태발생개변병조망；뢰공등홍소억제료 AGS 세포적천이，차해억제작용여농도、시간상관，차재종농도위1μM 작용우 AGS 세포12 h 시，기대 AGS 세포천이적억제작용최명현。
Objective To observe the effects of different concentration of celastrol on cellular mor-phology and migration of AGS cells at different time points;to explore a better inhibitory effect of concentra-tion and time of celastrol on migration of AGS cells. Methods AGS cells were planted and cultured in 6 -well plate. When the adherent cell density reached 70 ~ 80% ,cell migration was manufactured by scratch ex-periment. Thereafter,cell morphology and cell migration were observed under microscope with different con-centration of celastrol of 5 μM、1 μM、0. 5 μM、0. 1 μM、0. 01 μM at indicated time points. Results 1. High concentration of celastrol cause severe changed in the cell morphology. 2. Celastrol inhibited AGS cell migra-tion,and its inhibitory effect on the migration velocity was concentration - dependent in a certain range. The higher the concentration of drug,the stronger effect it was. The same concentration of celastrol on AGS cells at different times had different effect inhibition of cell migration(P ﹤ 0. 05). At the same time the different con-centrations of celastrol on AGS cells had different effect inhibition of cell migration(P ﹤ 0. 05). Conclusion 1. High concentrations of celastrol cause changes in the cell morphology and cell apoptosis. 2. Celastrol in-hibited AGS cell migration,which is dependent on the concentration and action time. The inhibitory effect of celastrol on AGS cell migration is most obvious under final concentration of 1 μM at 12 h.