世界中西医结合杂志
世界中西醫結閤雜誌
세계중서의결합잡지
WORLD JOURNAL OF TRADITIONAL CHINESE AND WESTERN MEDICINE
2015年
5期
632-635
,共4页
中药复方%痰风理论论治%ApoE 基因敲除小鼠%AS%不稳定斑块%HSP70%PPAR - γ
中藥複方%痰風理論論治%ApoE 基因敲除小鼠%AS%不穩定斑塊%HSP70%PPAR - γ
중약복방%담풍이론론치%ApoE 기인고제소서%AS%불은정반괴%HSP70%PPAR - γ
Chinese herbal compound%Searching wind and expectorant phlegm%ApoE KnocKout mice%AS%Unstable plaque%HSP70%PPAR - γ
目的：观察搜风祛痰中药复方稳斑汤对 ApoE 基因敲除小鼠动脉粥样硬化（AS）不稳定斑块的作用及机制。方法6～8周龄 ApoE 基因缺陷鼠，高脂饲料喂养13周后造出 AS 不稳定斑块模型，同时将正常 C57BL/6J 小鼠10只正常饲料喂养13周，设为空白对照组。各组分别给予相应药物干预1个月，麻醉处死小鼠取主动脉组织 HE 染色后在光学显微镜下进行病理学观察，然后采用免疫组化和 RT - PCR 技术检测 HSP70和 PPAR -γ的表达水平。结果与空白对照组比较，模型对照组 HSP70和 PPAR -γ明显下降（P ﹤0.01）；与模型对照组比较，中药低、中剂量组 HSP70和 PPAR-γ明显升增高（P ﹤0.05），中药高剂量组与西药组较模型对照组明显增高（P ﹤0.01），然而两者表达水平在中药高剂量组与西药组无明显差异（P ﹥0.05）。结论 ApoE 基因敲除小鼠 AS 不稳定斑块的形成与炎症因子密切相关，搜风祛痰中药复方稳斑汤可能通过调节 HSP70和 PPAR -γ的表达而干预 AS 斑块形成及破裂，为 AS 的防治开辟了一条新思路。
目的：觀察搜風祛痰中藥複方穩斑湯對 ApoE 基因敲除小鼠動脈粥樣硬化（AS）不穩定斑塊的作用及機製。方法6～8週齡 ApoE 基因缺陷鼠，高脂飼料餵養13週後造齣 AS 不穩定斑塊模型，同時將正常 C57BL/6J 小鼠10隻正常飼料餵養13週，設為空白對照組。各組分彆給予相應藥物榦預1箇月，痳醉處死小鼠取主動脈組織 HE 染色後在光學顯微鏡下進行病理學觀察，然後採用免疫組化和 RT - PCR 技術檢測 HSP70和 PPAR -γ的錶達水平。結果與空白對照組比較，模型對照組 HSP70和 PPAR -γ明顯下降（P ﹤0.01）；與模型對照組比較，中藥低、中劑量組 HSP70和 PPAR-γ明顯升增高（P ﹤0.05），中藥高劑量組與西藥組較模型對照組明顯增高（P ﹤0.01），然而兩者錶達水平在中藥高劑量組與西藥組無明顯差異（P ﹥0.05）。結論 ApoE 基因敲除小鼠 AS 不穩定斑塊的形成與炎癥因子密切相關，搜風祛痰中藥複方穩斑湯可能通過調節 HSP70和 PPAR -γ的錶達而榦預 AS 斑塊形成及破裂，為 AS 的防治開闢瞭一條新思路。
목적：관찰수풍거담중약복방은반탕대 ApoE 기인고제소서동맥죽양경화（AS）불은정반괴적작용급궤제。방법6～8주령 ApoE 기인결함서，고지사료위양13주후조출 AS 불은정반괴모형，동시장정상 C57BL/6J 소서10지정상사료위양13주，설위공백대조조。각조분별급여상응약물간예1개월，마취처사소서취주동맥조직 HE 염색후재광학현미경하진행병이학관찰，연후채용면역조화화 RT - PCR 기술검측 HSP70화 PPAR -γ적표체수평。결과여공백대조조비교，모형대조조 HSP70화 PPAR -γ명현하강（P ﹤0.01）；여모형대조조비교，중약저、중제량조 HSP70화 PPAR-γ명현승증고（P ﹤0.05），중약고제량조여서약조교모형대조조명현증고（P ﹤0.01），연이량자표체수평재중약고제량조여서약조무명현차이（P ﹥0.05）。결론 ApoE 기인고제소서 AS 불은정반괴적형성여염증인자밀절상관，수풍거담중약복방은반탕가능통과조절 HSP70화 PPAR -γ적표체이간예 AS 반괴형성급파렬，위 AS 적방치개벽료일조신사로。
Objective To observe the effect and mechanism of Chinese herbal compound on ApoE KnocKout mice animal models of unstable plaque AS. Methods Six to eight weeKs old ApoE gene - deficient mice were fed with high fat diet for 13 weeKs to establish atherosclerosis(atherosclerosis,AS)unstable plaque animal models,while 10 normal C57BL/ 6J mice were fed with normal diet for 13 weeKs to establish as the blanK control. Then they were given low dose,medium - dose,high - dose of Chinese herbal compound and western medicine for a month. At the same time,the model group was fed by normal sodium for one month. Mice were executed and the anesthetic and fetch aortic tissue were then observed under an optical microscope pathol-ogy after HE staining. Immunohistochemistry and RT - PCR technique were used to detect the expression levels of HSP70 and PPAR -γ. Results Compared with the control group,the expression level of HSP70 and PPAR- γ in model control group decreased significantly(P ﹤0. 01);compared with the model control group,the ex-pression level of HSP70 and PPAR - γ in Chinese herbal compound low dose group significantly increased(P ﹤0. 05);compared with the model control group,the expression level in Chinese herbal compound high - dose group and western medicine atorvastatin group was significantly higher(P ﹤0. 01);however,the expression lev-els in the both two group had no significant difference(P ﹥0. 05). Conclusion ApoE KnocKout mice AS un-stable plaque formation is closely related to inflammatory cytoKines. Searching wind and expectorant phlegm tra-ditional Chinese medicine may stabilize plaques by regulating the expression of HSP70 and PPAR -γ and pro-moting intervention on the formation and rupture of AS plaque,which provides a new idea to prevent AS.
