CAJ | 학술논문

目的：观察菩人丹（PuRenDan）对2型糖尿病胰腺微循环损伤大鼠胰腺组织中血管内皮生长因子（VEGF）及其受体（VEGFR2/ p - VEGFR2）和血管生成抑制素（Angiostatin）、内皮细胞抑制素（Endostatin）的影响，探讨菩人丹修复胰腺微循环障碍的机制。方法通过高脂饲料喂养（12周）与尾静脉快速注射小剂量链脲佐菌素（30 mg/ Kg）的方法建立大鼠模型。在同批次正常大鼠中随机选15只为正常对照组，后将成模大鼠随机分为：模型组、菩人丹组（1.77 g/ Kg）、降糖通脉片组（0.42 g/Kg）和二甲双胍组（0.14 g/ Kg），每组15只。各组大鼠每天灌胃1次，正常对照组和模型组给予等剂量蒸馏水，连续给药4周后，提取各组大鼠胰腺组织总蛋白质，采用 Western blot 法测定各组大鼠胰腺组织中 VEGF 及其受体 VEGFR2的蛋白质表达和磷酸化水平，以及 Angiostatin、Endostatin 的蛋白质表达水平。结果与正常对照组相比，模型组胰腺组织 VEGF 蛋白质表达水平和受体 VEGFR2磷酸化水平明显降低（P ﹤0.01），而 Angiostatin、Endostatin 蛋白质表达水平显著增高（P ﹤0.01）；菩人丹干预后，VEGF 蛋白质表达水平和受体 VEGFR2磷酸化水平均显著上调（P ﹤0.01），而 Angiostatin、Endosta-tin 蛋白质表达则被抑制（P ﹤0.01）。其与阳性对照药降糖通脉片和二甲双胍作用相当。结论菩人丹通过上调血管生成促进因子 VEGF 蛋白质表达及其受体 VEGFR2磷酸化，抑制血管生成抑制因子Angiostatin、Endostatin 的蛋白质表达，促进糖尿病状态下胰腺组织微血管新生，进而改善胰腺微循环障碍，发挥其对胰岛β细胞的保护、修复作用。
목적：관찰보인단（PuRenDan）대2형당뇨병이선미순배손상대서이선조직중혈관내피생장인자（VEGF）급기수체（VEGFR2/ p - VEGFR2）화혈관생성억제소（Angiostatin）、내피세포억제소（Endostatin）적영향，탐토보인단수복이선미순배장애적궤제。방법통과고지사료위양（12주）여미정맥쾌속주사소제량련뇨좌균소（30 mg/ Kg）적방법건립대서모형。재동비차정상대서중수궤선15지위정상대조조，후장성모대서수궤분위：모형조、보인단조（1.77 g/ Kg）、강당통맥편조（0.42 g/Kg）화이갑쌍고조（0.14 g/ Kg），매조15지。각조대서매천관위1차，정상대조조화모형조급여등제량증류수，련속급약4주후，제취각조대서이선조직총단백질，채용 Western blot 법측정각조대서이선조직중 VEGF 급기수체 VEGFR2적단백질표체화린산화수평，이급 Angiostatin、Endostatin 적단백질표체수평。결과여정상대조조상비，모형조이선조직 VEGF 단백질표체수평화수체 VEGFR2린산화수평명현강저（P ﹤0.01），이 Angiostatin、Endostatin 단백질표체수평현저증고（P ﹤0.01）；보인단간예후，VEGF 단백질표체수평화수체 VEGFR2린산화수평균현저상조（P ﹤0.01），이 Angiostatin、Endosta-tin 단백질표체칙피억제（P ﹤0.01）。기여양성대조약강당통맥편화이갑쌍고작용상당。결론보인단통과상조혈관생성촉진인자 VEGF 단백질표체급기수체 VEGFR2린산화，억제혈관생성억제인자Angiostatin、Endostatin 적단백질표체，촉진당뇨병상태하이선조직미혈관신생，진이개선이선미순배장애，발휘기대이도β세포적보호、수복작용。
Objective To observe the impacts of puren pills on VEGF and its receptor(VEGFR2 / p- VEGFR2),Angiostatin and Endostatin in the rats of pancreas micro - circulatory damage of T2DM so as to explore the mechanism of puren pills for pancreas micro - circulatory disturbance. Methods The feeding with high fat diet(12 weeKs)and the rapid injection with streptozotocin of small dose(30 mg/ Kg)via the vein on the tail were used to establish rat model. Among the normal rats of same batch,15 rats were selected ran-domly to set up a normal control group. The modeled rats were randomized into a model group,a puren pills group(1. 77 g/ Kg),a jiangtang tongmai tablets group(0. 42 g/ Kg)and a metformin group(0. 14 g/ Kg),15 rats in each one. The gavage was given once every day in the rats of each group. The stilled water of same dose was used in the normal control group and the model group. The medication was given continuously for 4 weeKs. Af-terwards,the total protein was collected from pancreas tissue in the rats of each group. Western blot method was adopted to determine the protein expressions of VEGF and VEGFR2 and the level of phosphorylation,as well as the protein expressions of Angiostatin and Endostatin. Results Compared with the normal control group, the protein expression of VEGF in pancreas tissue and the phosphorylation level of VEGFR2 were reduced apparently(P ﹤ 0. 01),and the protein expressions of Angiostatin and Endostatin were increased significantly (P ﹤ 0. 01). After the intervention with puren pills,the protein expression of VEGF in pancreas tissue and the phosphorylation level of VEGFRs were up - regulated significantly(P ﹤ 0. 01)and the protein expressions of Angiostatin and Endostatin were inhibited(P ﹤ 0. 01). The effects were similar to those in the positive con-trol groups treated with jiangtang tongmai tablets and metformin. Conclusion Puren pills up - regulate the protein expression of VEGF in pancreas tissue and the phosphorylation level of VEGFR2,inhibit the expres-sions of Angiostatin and Endostatin and promote the microvascular neogenesis so as to relieve pancreas micro-circulatory disturbance and play the role of protective and repair effect to the islet beta cells.