CAJ | 학술논문

本试验旨在从山羊瘤胃中分离、筛选和鉴定单宁降解菌。从隆林山羊瘤胃采集内容物,采用单宁浓度耐受培养基进行初筛,再采用单宁酶活力鉴定培养基分离、筛选单宁降解菌,筛选出的细菌通过16S rDNA基因序列测定初步鉴定,将菌株在单宁浓度为0.5%、1%、1.5%的单宁酶诱导培养基中培养,测定酶活力。结果表明：共分离、筛选到4株降解单宁的细菌,根据16S rDNA基因序列分析结果,分别为产碱普罗威登斯菌( Providencia alcalifaciens)、克斯特菌( Kerstersia)、普罗威登斯菌( Providencia vermicola)和铜绿假单胞菌( Pseudomonas aeruginosa)。经单宁酶诱导培养基培养后,Kerstersia的胞内单宁酶活力高于其他3株菌,而其他3株菌的酶活力较为接近；Kerstersia、Providencia vermicola和Pseudomonas aeruginosa经1%单宁浓度诱导的单宁酶活力均高于经0.5%和1.5%单宁浓度诱导( P<0.05)。本试验成功从山羊瘤胃中筛选、分离、鉴定出4株单宁降解菌,且它们均表现出较高的单宁酶活力。
본시험지재종산양류위중분리、사선화감정단저강해균。종륭림산양류위채집내용물,채용단저농도내수배양기진행초사,재채용단저매활력감정배양기분리、사선단저강해균,사선출적세균통과16S rDNA기인서렬측정초보감정,장균주재단저농도위0.5%、1%、1.5%적단저매유도배양기중배양,측정매활력。결과표명：공분리、사선도4주강해단저적세균,근거16S rDNA기인서렬분석결과,분별위산감보라위등사균( Providencia alcalifaciens)、극사특균( Kerstersia)、보라위등사균( Providencia vermicola)화동록가단포균( Pseudomonas aeruginosa)。경단저매유도배양기배양후,Kerstersia적포내단저매활력고우기타3주균,이기타3주균적매활력교위접근；Kerstersia、Providencia vermicola화Pseudomonas aeruginosa경1%단저농도유도적단저매활력균고우경0.5%화1.5%단저농도유도( P<0.05)。본시험성공종산양류위중사선、분리、감정출4주단저강해균,차타문균표현출교고적단저매활력。
This study was conducted to isolate, screen and identify several strains of tannin degradable bacteria in rumen of goats. Ruminal contents was collected from goats, and were preliminary screened by tolerance cul-ture medium with different tannic concentrations, then strains of tannin degradable bacteria were isolated, screened by tannase identification culture mediums. The screened strains were firstly identified by the methods of 16S rDNA gene sequencing, and then were cultured on tannase inductive culture mediums with different tan-nin concentrations ( 0. 5%, 1% and 1. 5%, respectively ) for the analysis of enzyme activity. The results showed as follows: four strains of tannin degradable bacteria were isolated and screened. According to the re-sults of 16S rDNA gene sequencing, the strains were Providencia alcalifaciens, Kerstersia, Providencia Ver-micola and Pseudomonas aeruginosa, respectively. After cultured on tannase inductive culture medium, the in-tra-cellular tannase activity of Kerstersia was higher than that of the other three strains, which was pretty close to each other; after inducted by 1.5% tannin, the activity was significantly higher than that inducted by 0.5%and 1% tannin ( P<0.05) . In conclusion, four strains with high enzyme activity of tannin degradable bacteria in rumen of goats was successfully isolated, screened and identified.