CAJ | 학술논문

为研究饲粮中添加单一剂量黄曲霉毒素 B1( AFB1)后奶山羊乳中黄曲霉毒素 M1(AFM1)的分泌规律及AFB1转化为AFM1的转化率,选择3只健康、体重接近的泌乳中期关中奶山羊,每只羊饲喂1 mg AFB1。饲喂后0、4、8、24、32、48、56、72、80、96、104及120 h采集羊乳,测定AFM1含量。结果表明：1)饲喂AFB1前(0 h),羊乳中未检出AFM1,但是饲喂AFB1后4 h,在收集的羊乳中均检出了AFM1；2)3只试验在羊乳中AFM1含量存在差异,但是其分泌规律非常相似,均呈先升高后急剧下降再缓慢下降的趋势；3)羊乳中 AFM1的峰值出现在饲喂AFB1后4~8 h；4)羊乳中 AFM1平均含量在56 h 时为0.39μg/kg,低于我国限量值(0.5μg/kg),到120 h平均含量为0.02μg/kg,低于欧盟限量值(0.05μg/kg)；5)各时间点AFB1转化为AFM1的最大平均转化率为0.19%,出现在4 h；6)饲喂AFB1后24 h内的AFM1平均转化率之和占AFM1总转化率的81%。结果提示,AFM1在奶山羊乳中的排出时间主要集中在采食AFB1后的24 h内。
위연구사량중첨가단일제량황곡매독소 B1( AFB1)후내산양유중황곡매독소 M1(AFM1)적분비규률급AFB1전화위AFM1적전화솔,선택3지건강、체중접근적비유중기관중내산양,매지양사위1 mg AFB1。사위후0、4、8、24、32、48、56、72、80、96、104급120 h채집양유,측정AFM1함량。결과표명：1)사위AFB1전(0 h),양유중미검출AFM1,단시사위AFB1후4 h,재수집적양유중균검출료AFM1；2)3지시험재양유중AFM1함량존재차이,단시기분비규률비상상사,균정선승고후급극하강재완만하강적추세；3)양유중 AFM1적봉치출현재사위AFB1후4~8 h；4)양유중 AFM1평균함량재56 h 시위0.39μg/kg,저우아국한량치(0.5μg/kg),도120 h평균함량위0.02μg/kg,저우구맹한량치(0.05μg/kg)；5)각시간점AFB1전화위AFM1적최대평균전화솔위0.19%,출현재4 h；6)사위AFB1후24 h내적AFM1평균전화솔지화점AFM1총전화솔적81%。결과제시,AFM1재내산양유중적배출시간주요집중재채식AFB1후적24 h내。
The aim of this study was to determine the excretion patterns and carry-over rate of aflatoxin M1 (AFM1) in milk of dairy goats fed a single dose of aflatoxin B1(AFB1). Three healthy Guanzhong dairy goats in mid-lactation with similar body weight were administered 1 mg of AFB1. Milk samples were collected after 0, 4, 8, 24, 32, 48, 56, 72, 80, 96, 104 and 120 h of AFB1 administration to determine AFM1 content. The results showed as follows:1) AFM1 was not detected in milk before AFB1 administration (0 h), but was detected in the first milking following AFB1 administration (4 h);2) the excretion pattern of AFM1 content in milk was very similar in all the experimental goats even if the values differed among animals;3) The peak val-ue for AFM1 content in milk was observed in milk collected at 4 and 8 h after AFB1 administration;4) AFM1 average content in milk was 0.39 μg/kg at 56 h and 0.02 μg/kg at 120 h, which moved below the Chinese limit (0.5 μg/kg)and the European Union limit (0.05 μg/kg), respectively;5) the maximum value of aver-age carry-over rate of AFB1 into AFM1 in milk was 0.19% which appeared at 4 h;6) the sum of average car-ry-over rate at different time point in 24 h was 81% of total average carry-over rate after AFB1 administration. In conclusion, AFM1 is mostly excreted in milk of dairy goats in 24 h after AFB1 administration.