CAJ | 학술논문

本试验旨在研究递增30%注射脂多糖( LPS)诱导仔猪慢性免疫应激的效果及对仔猪生长性能、营养物质表观消化率的影响。选取16头断奶仔猪,随机分为2组(对照组、LPS组),单笼饲养。 LPS组隔天静脉注射LPS,初始注射剂量80μg/kg BW,随后逐次递增30%,对照组注射相应剂量的生理盐水,试验期15 d。研究发现：仔猪注射LPS第1、5天血浆白细胞介素-1β和皮质醇含量极显著高于对照组(P<0.01),第9天血浆皮质醇有高于对照组的趋势(P=0.10),整个试验期内血浆结合珠蛋白含量显著高于对照组( P<0.05)。与对照组相比,注射LPS仔猪在试验的3个阶段(1~4 d、5~8 d和9~15 d)平均日增重分别降低了40.00%( P<0.01)、29.52%(P<0.05)和19.30%(P<0.05),平均日采食量分别降低了25.09%(P=0.01)、23.15%( P<0.05)和19.47%( P<0.05),增重耗料比与对照组无显著差异( P>0.05)。与对照组相比,注射LPS仔猪饲粮干物质表观消化率下降了5.43%(P<0.05),能量表观消化率有降低的趋势(P=0.06),粗脂肪和粗蛋白质表观消化率略有降低(P>0.10)。试验结果表明,递增30%注射LPS成功诱导仔猪慢性免疫应激,降低了仔猪平均日采食量和营养物质表观消化率,抑制了仔猪生长。
본시험지재연구체증30%주사지다당( LPS)유도자저만성면역응격적효과급대자저생장성능、영양물질표관소화솔적영향。선취16두단내자저,수궤분위2조(대조조、LPS조),단롱사양。 LPS조격천정맥주사LPS,초시주사제량80μg/kg BW,수후축차체증30%,대조조주사상응제량적생리염수,시험기15 d。연구발현：자저주사LPS제1、5천혈장백세포개소-1β화피질순함량겁현저고우대조조(P<0.01),제9천혈장피질순유고우대조조적추세(P=0.10),정개시험기내혈장결합주단백함량현저고우대조조( P<0.05)。여대조조상비,주사LPS자저재시험적3개계단(1~4 d、5~8 d화9~15 d)평균일증중분별강저료40.00%( P<0.01)、29.52%(P<0.05)화19.30%(P<0.05),평균일채식량분별강저료25.09%(P=0.01)、23.15%( P<0.05)화19.47%( P<0.05),증중모료비여대조조무현저차이( P>0.05)。여대조조상비,주사LPS자저사량간물질표관소화솔하강료5.43%(P<0.05),능량표관소화솔유강저적추세(P=0.06),조지방화조단백질표관소화솔략유강저(P>0.10)。시험결과표명,체증30%주사LPS성공유도자저만성면역응격,강저료자저평균일채식량화영양물질표관소화솔,억제료자저생장。
This experiment was conducted to study the effects of gradually increasing 30% of lipopolysaccha-ride ( LPS) by injecting on the chronic immunological stress and on growth performance and nutrient apparent digestibility of piglets. Sixteen weaned piglets were randomly allotted into 2 groups ( control and LPS groups) , and were individually housed in metabolic cages. Saline ( control) or LPS were injected at 48 h intervals with an initial dosage of 80 μg/kg BW, increased by 30% at each following injections. The experiment lasted for 15 d. The results showed that the contents of interleukin-1β and cortisol in plasma of the LPS group were sig-nificantly higher than those of control group on days 1 and 5 ( P<0.01) , and there was a trend that the content of cortisol in plasma of LPS group was higher than that of control group ( P=0.10) . Moreover, the content of haptoglobin in plasma was significantly higher than that of control group during the whole experimental period ( P<0.05) . Compared with the control group, during three phases of this experiment ( from 1 to 4 days, 5 to 8 days and 9 to 15 days) , the average daily gain of LPS group was decreased by 40.00% ( P<0.01) , 29.52%( P<0.05) and 19.30% ( P<0.05) , respectively. For the average daily feed intake was decreased by 25.09%( P=0.01) , 23.15% ( P<0.05) and 19.47% ( P<0.05) , respectively. At last, no significant difference was found in gain to feed ratio ( P>0.05) . Compared with control group, the apparent digestibility of dry matter in LPS group was decreased by 5.43% ( P<0.05) , and gross energy apparent digestibility had a decreasing trend ( P=0.06) , apparent digestibility of crude protein and ether extract were decreased slightly ( P>0.10) . In con-clusion, increasing of 30% injection of LPS successfully induces the chronic immunological stress, and reduces feed intake and nutrient apparent digestibility of piglet, as well as inhibites the growth of piglets.